Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China.
Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China; School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, China.
Anal Chim Acta. 2022 Sep 8;1225:340241. doi: 10.1016/j.aca.2022.340241. Epub 2022 Aug 11.
Spatially-resolved profiling of tissue monosaccharides not only gives an insight into the spatial heterogeneity of monosaccharides, but also helps to decipher the possible roles of monosaccharides in biological processes. Here, we develop an on-tissue derivatization method, coupled with matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) to image and quantify the aldose and ketose isomers of monosaccharide in biological tissues. A new derivatization reagent, 1-naphthaleneacethydrazide (NAH) was synthesized for the on-tissue derivatization of monosaccharides, and it significantly enhanced the imaging sensitivity of monosaccharides. Moreover, the NAH-derivatized aldose and ketose can generate isomer-specific diagnostic ions during MALDI-MS/MS analysis, and thus paves way for the isomer-specific MS imaging of aldose and ketose monosaccharides. On this basis, we further constructed a quantitative MALDI-MS imaging model based on isomer-specific diagnostic ions, and calculated the expression contents of aldose and ketose monosaccharide isomers in different tissue regions of carrot section. We expect that the development of this method should provide more precise view on the spatial distributions and contents of different monosaccharides in heterogeneous biological tissues.
组织中单糖的空间分辨分析不仅可以深入了解单糖的空间异质性，还有助于揭示单糖在生物过程中的可能作用。在这里，我们开发了一种在组织上进行衍生化的方法，结合基质辅助激光解吸/电离质谱（MALDI-MS）来对生物组织中单糖的醛糖和酮糖异构体进行成像和定量分析。我们合成了一种新的衍生化试剂 1-萘乙二酰亚胺（NAH），用于单糖的在组织上衍生化，它显著提高了单糖的成像灵敏度。此外，NAH 衍生化的醛糖和酮糖在 MALDI-MS/MS 分析中会产生具有异构体特异性的诊断离子，从而为醛糖和酮糖单糖的异构体特异性 MS 成像铺平了道路。在此基础上，我们进一步构建了基于异构体特异性诊断离子的定量 MALDI-MS 成像模型，并计算了胡萝卜切片不同组织区域中醛糖和酮糖单糖异构体的表达含量。我们期望这种方法的发展能够为异质生物组织中不同单糖的空间分布和含量提供更精确的观察。
