Department of Agro-Food Resources, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, 55365, Republic of Korea.
Department of Food Science and Technology, Jeonbuk National University, Jeonju, 54896, Republic of Korea.
Sci Rep. 2022 Aug 29;12(1):14678. doi: 10.1038/s41598-022-18226-4.
Most previous studies have been focused on isoflavone profile with biological activities from soybean seed and its related products. However, in the present study, eighty-three flavonoid derivatives (55 flavonols, 9 flavones and 19 isoflavones) were comprehensively identified and quantified from young leaves of 21 core-collected soybean cultivars based on ultra-performance liquid chromatography-diode array detector with quadrupole time of flight/mass spectrometry (UPLC-DAD-QToF/MS). Among total flavonoids from soybean leaves (SLs), the abundant flavonols (83.6%) were primarily composed of di- and tri- glycosides combined to the aglycones (K, kaempferol; Q, quercetin; I, isorhamnetin). Particularly, K-rich SLs (yellow coated seed), Nongrim 51 (breeding line) and YJ208-1 (landrace) contained mainly kaempferol 3-O-(2″-O-glucosyl-6″-O-rhamnosyl)galactoside and 3-O-(2″,6″-di-O-rhamnosyl)galactoside, and were expected to be superior cultivars by their higher flavonoids. Besides, the new tri-I-glycosides (soyanins I-V) were presented as predominant components in Junyeorikong (landrace, black). Thus, this study suggest that the SLs can be considered as valuable edible resources due to their rich flavonoids. Also, these detailed profiles will support breeding of superior varieties with excellent biological activities as well as relationship with seed anthocyanins production, and contribute to perform metabolomics approach to investigate the changes of SLs flavonols during the leaf growth and fermentation in further research.
大多数先前的研究都集中在大豆种子及其相关产品的生物活性的异黄酮谱上。然而，在本研究中，基于超高效液相色谱-二极管阵列检测-四极杆飞行时间/质谱联用仪（UPLC-DAD-QToF/MS），从 21 个核心收集的大豆品种的幼叶中全面鉴定和定量了 83 种黄酮类衍生物（55 种黄酮醇、9 种黄酮和 19 种异黄酮）。在大豆叶总黄酮（SLs）中，丰富的黄酮醇（83.6%）主要由二糖苷和三糖苷与苷元（K，山奈酚；Q，槲皮素；I，异鼠李素）组成。特别是，富含 K 的 SLs（黄皮种子）、Nongrim 51（育成系）和 YJ208-1（地方品种）主要含有山奈酚 3-O-（2″-O-葡萄糖基-6″-O-鼠李糖苷）半乳糖苷和 3-O-（2″，6″-二-O-鼠李糖苷）半乳糖苷，并且由于其较高的类黄酮含量，预计是较好的品种。此外，新的三-I-糖苷（大豆苷元 I-V）作为 Junyeorikong（地方品种，黑色）的主要成分存在。因此，本研究表明，由于富含类黄酮，SLs 可以被视为有价值的食用资源。此外，这些详细的图谱将支持优良品种的选育，这些品种具有优异的生物活性，并与种子花青素的产生有关，并有助于在进一步的研究中采用代谢组学方法来研究 SLs 黄酮醇在叶片生长和发酵过程中的变化。
