College of Pharmacy, Duksung Women's University, Seoul 01369, Republic of Korea.
Department of Pediatrics, Hallym University College of Medicine, Hwaseong 18450, Republic of Korea.
J Microbiol Biotechnol. 2022 Sep 28;32(9):1186-1194. doi: 10.4014/jmb.2206.06023. Epub 2022 Aug 11.
The intake of probiotic lactic acid bacteria not only promotes digestion through the microbiome regulated host intestinal metabolism but also improves diseases such as irritable bowel syndrome and inflammatory bowel disease, and suppresses pathogenic harmful bacteria. This investigation aimed to evaluate the immunomodulatory effects in intestinal epithelial cells and to study the clinical efficacy of the selected the and groups. The physiological and biochemical properties were characterized, and immunomodulatory activity was measured against pathogenic bacteria. In order to find out the mechanism of inflammatory action of the eight viable and sonicated spp., we tried to confirm the changes in the pro-inflammatory cytokines (TNF-α, interleukin (IL)-6, IL-12) and anti-inflammatory cytokine (IL-10), and chemokines, (monocyte chemoattractant protein-1, IL-8) and inflammatory enzymatic mediator (nitric oxide) against ATCC 29212 infection in Caco-2 cells and RAW 264.7 cells. The clinical efficacy of the selected and group was studied as a probiotic adjuvant for acute diarrhea in children by oral administration. The results showed significant immunomodulatory effects on the expression levels of TNF-α, IL-6, IL-12, MCP-1, IL-8 and NO, in sonicated extracts and viable bifidobacteria. Moreover, each of the strains was found to react more specifically to different cytokines. However, treatment with sonicated extracts showed a more significant effect compared to treatment with the viable bacteria. We suggest that probiotics functions should be subdivided according to individual characteristics, and that personalized probiotics should be designed to address individual applications.
摄入益生菌乳酸杆菌不仅可以通过微生物组调节宿主肠道代谢来促进消化，还可以改善肠易激综合征和炎症性肠病等疾病，并抑制病原有害细菌。本研究旨在评估其对肠道上皮细胞的免疫调节作用，并研究所选[具体分组]组的临床疗效。对其生理生化特性进行了表征，并针对病原菌进行了免疫调节活性测定。为了找出这 8 株活菌和超声[具体分组]的炎症作用机制，我们试图证实促炎细胞因子（TNF-α、白细胞介素 (IL)-6、IL-12）和抗炎细胞因子（IL-10）、趋化因子（单核细胞趋化蛋白-1、IL-8）和炎症酶介质（一氧化氮）在 Caco-2 细胞和 RAW 264.7 细胞中针对 ATCC 29212 感染的变化。通过口服给药，研究了所选[具体分组]组作为儿童急性腹泻的益生菌佐剂的临床疗效。结果表明，超声[具体分组]提取物和活菌双歧杆菌对 TNF-α、IL-6、IL-12、MCP-1、IL-8 和 NO 的表达水平均有显著的免疫调节作用。此外，发现每种[具体分组]菌株对不同的细胞因子都有更特异的反应。然而，与活菌处理相比，超声[具体分组]提取物处理显示出更显著的效果。我们建议根据个体特征对益生菌功能进行细分，并设计个性化益生菌以满足个体应用。
