Pei Ying-Hong, Yan Ning-Ning, Zhang Hui-Fang, Zhang Shu-Ting, Tang Zi-Zhong, Huang Yan, Chen Tao, Feng Shi-Ling, Ding Chun-Bang, Yuan Shu, Yuan Ming
College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Yucheng District, Ya'an, Sichuan, China.
College of Resources Science, Sichuan Agricultural University, Sichuan, China.
Chem Biodivers. 2022 Oct;19(10):e202200156. doi: 10.1002/cbdv.202200156. Epub 2022 Sep 16.
Alsophila spinulosa, as a rare tree fern with potential medicinal value, has attracted extensive attention. Herein, the physicochemical properties, antioxidant and anti-aging activities of polysaccharide from A. spinulosa leaf (ALP) were investigated. ALP was composed of galactose, arabinose, glucose, rhamnose, galacturonic acid, mannose, and fucose. (1→), (1→6), and (1→2) bond types were the primary glycosidic bond in ALP. Surprisingly, ALP displayed the wonderful activity of antioxidant and anti-aging, including excellent scavenging ability against DPPH and ABTS radicals in vitro; prolonging the life span, improving activity of antioxidative enzymes (SOD and CAT), and decreasing the level of ROS, MDA in Caenorhabditis elegans. Meanwhile, ALP promoted DAF-16 to move into the nuclear. Overall, our results illustrated that ALP could be further developed as a functional food ingredient.
桫椤作为一种具有潜在药用价值的珍稀树蕨，已引起广泛关注。在此，对桫椤叶多糖（ALP）的理化性质、抗氧化和抗衰老活性进行了研究。ALP由半乳糖、阿拉伯糖、葡萄糖、鼠李糖、半乳糖醛酸、甘露糖和岩藻糖组成。（1→）、（1→6）和（1→2）键型是ALP中的主要糖苷键。令人惊讶的是，ALP表现出出色的抗氧化和抗衰老活性，包括在体外对DPPH和ABTS自由基具有优异的清除能力；延长秀丽隐杆线虫的寿命，提高抗氧化酶（SOD和CAT）的活性，并降低其体内活性氧（ROS）、丙二醛（MDA）的水平。同时，ALP促进DAF-16进入细胞核。总体而言，我们的结果表明ALP可进一步开发为功能性食品成分。
