College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Beijing Key Laboratory of Flavor Chemistry, Beijing Technology and Business University (BTBU), Beijing 100048, China.
Int J Biol Macromol. 2021 Jan 31;168:152-162. doi: 10.1016/j.ijbiomac.2020.12.010. Epub 2020 Dec 7.
A novel polysaccharide from Chlorella pyrenoidosa (CPP) was separated and purified with the average molecular weight 15.8 kDa. It was composed of seven monosaccharides including mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose. FT-IR and NMR spectra analysis further revealed that CPP was an acidic polysaccharide consisting of β-L-Arap-(1→, →2)-α-L-Rhap-(1→, β-D-GlcpA-(1→, →4)-α-D-GalpA-(1→, →6)-β-D-Glcp-(1→, →3)-β-D-Manp-(1→, and →3, 6)-β-D-Galp-(1→. The CPP treatment could effectively prolong lifespan of Caenorhabditis elegans under the oxidative stress conditions and inhibit the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) as well as enhancing the level of superoxide dismutase (SOD). It could up-regulate the expressions of Daf-16 and Skn-1 genes via declining miR-48-3p, miR-48-5p, and miR-51-5p translocation. Moreover, 16S rRNA sequencing revealed that the CPP-enriched Faecalibacterium, Haemophilus, Vibrio, and Shewanella were strongly correlated with SOD, MDA, apoptosis, and ROS. These results indicated that CPP may be considered as a desired ingredient on regulating the aging and oxidative diseases.
从蛋白核小球藻中分离得到一种新型多糖（CPP），其平均分子量为 15.8 kDa。该多糖由七种单糖组成，包括甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖和阿拉伯糖。傅里叶变换红外光谱（FT-IR）和核磁共振（NMR）谱分析进一步表明，CPP 是一种酸性多糖，由β-L-Arap-(1→, →2)-α-L-Rhap-(1→、β-D-GlcpA-(1→、→4)-α-D-GalpA-(1→、→6)-β-D-Glcp-(1→、→3)-β-D-Manp-(1→和→3, 6)-β-D-Galp-(1→组成。CPP 处理可以有效延长秀丽隐杆线虫在氧化应激条件下的寿命，并抑制活性氧（ROS）和丙二醛（MDA）的积累，同时提高超氧化物歧化酶（SOD）的水平。它可以通过降低 miR-48-3p、miR-48-5p 和 miR-51-5p 的易位来上调 Daf-16 和 Skn-1 基因的表达。此外，16S rRNA 测序表明，CPP 富集的粪杆菌、嗜血杆菌、弧菌和希瓦氏菌与 SOD、MDA、凋亡和 ROS 呈强相关。这些结果表明，CPP 可能被认为是一种调节衰老和氧化疾病的理想成分。
