Department of Biosciences, Durham University, United Kingdom.
Centre for Life's Origins and Evolution, Department of Genetics, Evolution and Environment, University College London, London, WC1E 6BT, United Kingdom.
Genome Biol Evol. 2022 Sep 6;14(9). doi: 10.1093/gbe/evac133.
Polyclad flatworms are widely thought to be one of the least derived of the flatworm classes and, as such, are well placed to investigate evolutionary and developmental features such as spiral cleavage and larval diversification lost in other platyhelminths. Prostheceraeus crozieri, (formerly Maritigrella crozieri), is an emerging model polyclad flatworm that already has some useful transcriptome data but, to date, no sequenced genome. We have used high molecular weight DNA extraction and long-read PacBio sequencing to assemble the highly repetitive (67.9%) P. crozieri genome (2.07 Gb). We have annotated 43,325 genes, with 89.7% BUSCO completeness. Perhaps reflecting its large genome, introns were considerably larger than other free-living flatworms, but evidence of abundant transposable elements suggests genome expansion has been principally via transposable elements activity. This genome resource will be of great use for future developmental and phylogenomic research.
多歧盘虫被广泛认为是扁形动物门中最原始的类群之一，因此非常适合研究螺旋分裂和幼虫多样化等进化和发育特征，而这些特征在其他扁形动物门中已经丢失。 Prostheceraeus crozieri（以前称为 Maritigrella crozieri）是一种新兴的多歧盘虫模式生物，已经有一些有用的转录组数据，但迄今为止还没有测序的基因组。我们使用高分子量 DNA 提取和长读长 PacBio 测序来组装高度重复（67.9%）的 P. crozieri 基因组（2.07 Gb）。我们注释了 43,325 个基因，具有 89.7%的 BUSCO 完整性。也许反映了其庞大的基因组，内含子比其他自由生活的扁形动物大得多，但大量转座元件的证据表明，基因组的扩张主要是通过转座元件的活动。这个基因组资源将对未来的发育和系统基因组学研究非常有用。
