School of Materials Science and Engineering, Hefei University of Technology, Hefei 230009, China.
Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
Analyst. 2022 Sep 26;147(19):4337-4347. doi: 10.1039/d2an01100j.
Mercury ions are some of the most toxic heavy metal ions widely distributed in the environment. Hg cannot be degraded by microorganisms and can accumulate in the body through the food chain, posing a great threat to human health. Herein, a method combining surface-enhanced Raman scattering (SERS) with a lateral flow strip (LFS) (SERS-LFS) was developed for the rapid and quantitative analysis of Hg. The recognition of Hg by the LFS depends on a specific T-Hg-T strategy. Rhodamine 6G (R6G), as a SERS reporter, was assembled on the surface of Au@Ag core-shell nanoparticles, which served as an effective SERS substrate on both the T and C lines of the LFS. Using this SERS-LFS, the presence of target Hg could be measured through the appearance of an orange color on the T line of the LFS. Furthermore, with the direct measurement of SERS on the T lines of the LFS, the SERS signal could be collected even at a low concentration of Hg, at which point the signal on the T lines could not be observed. Based on the intensities of the characteristic peak at 1513 cm of R6G collected on the T lines of the LFS, a linear relationship was obtained in the range of 0.01 nM to 1 nM, and the detection limit of the LFS was greatly improved by at least 20 times over the colorimetric results of the traditional LFS based on naked-eye observation. More importantly, this SERS-LFS did not affect the intrinsic simplicity of the LFS. This developed SERS-LFS holds great potential in practical routine screening in food safety and environmental monitoring.
汞离子是环境中分布最广泛的有毒重金属离子之一。Hg 不能被微生物降解，并且可以通过食物链在体内积累，对人类健康构成巨大威胁。在此，开发了一种将表面增强拉曼散射 (SERS) 与横向流动条带 (LFS) 相结合的方法 (SERS-LFS)，用于 Hg 的快速定量分析。LFS 对 Hg 的识别取决于特定的 T-Hg-T 策略。作为 SERS 报告器的罗丹明 6G (R6G) 被组装在 Au@Ag 核壳纳米粒子的表面上，作为 LFS 的 T 和 C 线上的有效 SERS 基底。使用这种 SERS-LFS，可以通过 LFS 的 T 线上出现橙色来测量目标 Hg 的存在。此外，通过直接在 LFS 的 T 线上进行 SERS 测量，可以在 Hg 的低浓度下收集 SERS 信号，此时在 T 线上无法观察到信号。基于在 LFS 的 T 线上收集的 R6G 的特征峰在 1513 cm 处的强度，在 0.01 nM 至 1 nM 的范围内获得了线性关系，并且与基于肉眼观察的传统 LFS 的比色结果相比，LFS 的检测限至少提高了 20 倍。更重要的是，这种 SERS-LFS 并没有影响 LFS 的固有简单性。这种开发的 SERS-LFS 在食品安全和环境监测的实际常规筛选中具有很大的潜力。
