坚韧的诱饵介导的心脏基因抑制。

Tough Decoy-Mediated Cardiac Gene Suppression.

机构信息

Division of Applied Medicine, School of Korean Medicine, Pusan National University, Yangsan, South Korea.

出版信息

Methods Mol Biol. 2022;2573:13-30. doi: 10.1007/978-1-0716-2707-5_2.

Abstract

MicroRNA (miRNA) is a small, non-coding RNA molecule (~22 nucleotides) that acts as a post-transcriptional gene regulator, primarily by inhibiting the translation of target mRNA transcripts or affecting cell mRNA stability. Since miRNAs are comprehensively involved in gene regulation, their abnormalities are associated with various human diseases, including cardiovascular disease. Additionally, targeted inhibition of disease-related miRNAs and their targets should have therapeutic potential. Therefore, this chapter describes the experimental steps for targeted inhibition of specific miRNAs using adenoviral vectorized tough decoys that efficiently silence miRNA function in cardiac cells.

摘要

微小 RNA（miRNA）是一种小的、非编码的 RNA 分子（约 22 个核苷酸），作为一种转录后基因调控因子，主要通过抑制靶 mRNA 转录本的翻译或影响细胞 mRNA 的稳定性来发挥作用。由于 miRNA 广泛参与基因调控，它们的异常与包括心血管疾病在内的各种人类疾病有关。此外，针对与疾病相关的 miRNA 及其靶标的抑制应该具有治疗潜力。因此，本章描述了使用腺病毒载体化的坚韧诱饵靶向抑制特定 miRNA 的实验步骤，该诱饵能够有效地抑制心脏细胞中的 miRNA 功能。