Department of Agricultural Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan; Genomics Research Center, Academia Sinica, No. 128, Sec. 2, Academia Rd., Taipei 11529, Taiwan.
Genomics Research Center, Academia Sinica, No. 128, Sec. 2, Academia Rd., Taipei 11529, Taiwan.
J Chromatogr A. 2022 Sep 13;1680:463445. doi: 10.1016/j.chroma.2022.463445. Epub 2022 Aug 24.
Polysaccharides are biopolymers known to possess various bioactivities. Because of their molecular complexity, the structural characterization of polysaccharides remains challenging, and difficult to be completed with a single analytical method. In this study, a novel approach for the characterization of linkages and anomeric configuration of polysaccharides was proposed. Based on ion mobility-mass spectrometry (IM-MS), a database containing 5 glucotriose standards was set up. Information about the arrival time distribution and fragmentation patterns of these standards were included. The method was validated by three commercially available purified polysaccharides, namely laminarin, dextrin, and dextran, each having distinct connectivity and configuration of the glycosidic bonds. Lastly, the method was successfully applied to analyze polysaccharides prepared from three medicinal mushrooms, namely Xylaria nigripes, Grifola frondosa, and Laetiporus sulphureus. The results showed that water-soluble non-digestible polysaccharides of X. nigripes and G. frondosa were mainly composed of (1→3)-β-glucan, while that of L. sulphureus was composed of (1→3)-ɑ-glucan. The present method has the advantages of being simple in sample preparation and short analysis time.
多糖是具有多种生物活性的生物聚合物。由于其分子结构复杂，多糖的结构表征仍然具有挑战性，并且难以仅用一种分析方法完成。在这项研究中，提出了一种用于多糖键合和端基构型表征的新方法。基于离子淌度-质谱（IM-MS），建立了包含 5 个葡萄糖三糖标准品的数据库，其中包含了这些标准品的到达时间分布和碎片模式信息。该方法通过三种市售的纯化多糖（即昆布多糖、糊精和葡聚糖）进行了验证，它们具有不同的糖苷键连接性和构型。最后，该方法成功地应用于分析三种药用蘑菇（即密褶黑菇、灰树花和硫磺菌）制备的多糖。结果表明，黑菇和灰树花的水溶性不可消化多糖主要由（1→3）-β-葡聚糖组成，而硫磺菌的多糖由（1→3）-α-葡聚糖组成。本方法具有样品制备简单和分析时间短的优点。
