Naylor Cameron N, Nagy Gabe
Department of Chemistry, University of Utah, Salt Lake City, Utah, USA.
Mass Spectrom Rev. 2024 Aug 1. doi: 10.1002/mas.21902.
Recently, ion mobility spectrometry-mass spectrometry (IMS-MS) has become more readily incorporated into various omics-based workflows. These growing applications are due to developments in instrumentation within the last decade that have enabled higher-resolution ion mobility separations. Two such platforms are the cyclic (cIMS) and structures for lossless ion manipulations (SLIM), both of which use traveling wave ion mobility spectrometry (TWIMS). High-resolution separations achieved with these techniques stem from the drastically increased pathlengths, on the order of 10 s of meters to >1 km, in both cIMS-MS and SLIM IMS-MS, respectively. Herein, we highlight recent developments and advances, for the period 2019-2023, in high-resolution traveling wave-based IMS-MS through instrumentation, calibration strategies, hyphenated techniques, and applications. Specifically, we will discuss applications including CCS calculations in multipass IMS-MS separations, coupling of IMS-MS with chromatography, imaging, and cryogenic infrared spectroscopy, and isomeric separations of glycans, lipids, and other small metabolites.
最近，离子淌度光谱-质谱联用技术（IMS-MS）已更易于融入各种基于组学的工作流程中。这些应用的不断增加得益于过去十年仪器设备的发展，这些发展使得离子淌度分离具有更高的分辨率。其中两个平台是循环离子淌度光谱（cIMS）和无损离子操纵结构（SLIM），它们都采用行波离子淌度光谱（TWIMS）。通过这些技术实现的高分辨率分离源于cIMS-MS和SLIM IMS-MS中路径长度的大幅增加，分别达到数十米至超过1千米的量级。在此，我们重点介绍2019年至2023年期间，基于高分辨率行波的IMS-MS在仪器设备、校准策略、联用技术及应用方面的最新进展。具体而言，我们将讨论包括多通道IMS-MS分离中碰撞截面（CCS）计算、IMS-MS与色谱、成像及低温红外光谱联用，以及聚糖、脂质和其他小代谢物的异构体分离等应用。
