School of Ocean Science and Technology, Dalian University of Technology, Panjin 124221, China.
School of Ocean Science and Technology, Dalian University of Technology, Panjin 124221, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Jan 5;284:121779. doi: 10.1016/j.saa.2022.121779. Epub 2022 Aug 27.
Online monitoring of antibiotics in the environment attracts more and more attention. Surface-enhanced Raman scattering (SERS) is a promising technique for the detection of trace amounts of antibiotics in the environment, which is fast, non-invasive and sensitive. To investigate the enrichment of trace amounts of antibiotics in water, polyethylene microplastics (PE MPs) were prepared as sorbents to simply concentrate enrofloxacin, ciprofloxacin hydrochloride monohydrate and triclosan in water, followed by the SERS measurement of antibiotics extract washed from MPs on an AgNPs@Si SERS substrate. Limit of detection of Rhodamine 6G is 2.1 × 10 M achieved from the AgNPs@Si SERS, indicating a high enhancement. The detection results show that SERS peaks of the antibiotics could be observed from the spectra of the extracts eluted from MPs, indicating MPs could adsorb and desorb antibiotics from water. Besides, for enrofloxacin and triclosan, the intensity of SERS measured from the MPs extracts are higher than that of directly from the spiked water, demonstrating the proposed method could lower the detectable concentration of hydrophobic antibiotics in water. Moreover, the proposed MPs sorbents combined with SERS method was applied to detect the antibiotics in real river water, with minimal detection of 10 M, 10 M, and 10 M achieved for enrofloxacin, ciprofloxacin hydrochloride monohydrate and triclosan, respectively. The proposed method provides a promising simple, rapid and low reagent consuming means for monitoring antibiotics in water.
在线监测环境中的抗生素越来越受到关注。表面增强拉曼散射（SERS）是一种很有前途的检测环境中痕量抗生素的技术，它具有快速、非侵入性和灵敏的特点。为了研究痕量抗生素在水中的富集情况，我们制备了聚乙烯微塑料（PE MPs）作为吸附剂，简单地浓缩水中的恩诺沙星、盐酸环丙沙星一水合物和三氯生，然后在 AgNPs@Si SERS 基底上对从 MPs 上洗脱的抗生素提取物进行 SERS 测量。通过 AgNPs@Si SERS 实现了 Rhodamine 6G 的检测限为 2.1×10-7 M，表明具有较高的增强效果。检测结果表明，从 MPs 洗脱的提取物的光谱中可以观察到抗生素的 SERS 峰，表明 MPs 可以从水中吸附和解吸抗生素。此外，对于恩诺沙星和三氯生，从 MPs 提取物中测量的 SERS 强度高于直接从加标水中测量的强度，表明所提出的方法可以降低水中疏水性抗生素的检测浓度。此外，我们将所提出的 MPs 吸附剂与 SERS 方法结合，用于检测实际河水样品中的抗生素，对于恩诺沙星、盐酸环丙沙星一水合物和三氯生，分别实现了 10-7 M、10-7 M 和 10-7 M 的最小检测限。该方法为监测水中抗生素提供了一种有前途的简单、快速和低试剂消耗的方法。
