College of Engineering, Nanjing Agricultural University, Nanjing 210031, China.
College of Engineering, Nanjing Agricultural University, Nanjing 210031, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 5;302:123056. doi: 10.1016/j.saa.2023.123056. Epub 2023 Jun 22.
Antibiotic residues in aquaculture environments disrupt the ecosystem balance and pose a potential hazard to human health when entering the food chain. Therefore, ultra-sensitive detection of antibiotics is necessary. In this study, a multifunctional FeO@mTiO@Ag core-shell nanoparticle (NP), synthesized using a layer-by-layer method, was demonstrated to be useful as an enhanced substrate for in-situ surface-enhanced Raman spectroscopy (SERS) detection of various quinolone antibiotics in aqueous environments. The results showed that the minimum detectable concentrations of the six investigated antibiotics were 1 × 10 mol/L (ciprofloxacin, danofloxacin, enoxacin, enrofloxacin, and norfloxacin) and 1 × 10 mol/L (difloxacin hydrochloride) under the enrichment and enhancement of FeO@mTiO@Ag NPs. Additionally, there was a good quantitative relationship between the antibiotics concentrations and SERS peak intensities within a certain detection range. The results of the spiked assay of actual aquaculture water samples showed that the recoveries of the six antibiotics ranged from 82.9% to 113.5%, with relative standard deviations ranging from 1.71% to 7.24%. In addition, FeO@mTiO@Ag NPs achieved satisfactory results in assisting the photocatalytic degradation of antibiotics in aqueous environments. This provides a multifunctional solution for low concentration detection and efficient degradation of antibiotics in aquaculture water.
养殖环境中的抗生素残留会破坏生态系统平衡，当其进入食物链时会对人类健康构成潜在威胁。因此，需要超灵敏地检测抗生素。在本研究中，我们使用层层自组装法合成了一种多功能的 FeO@mTiO@Ag 核壳纳米粒子（NP），该 NP 可用作增强衬底，用于原位表面增强拉曼光谱（SERS）检测水相环境中的各种喹诺酮类抗生素。结果表明，在 FeO@mTiO@Ag NPs 的富集和增强作用下，六种研究抗生素的最低检测浓度分别为 1×10-6 mol/L（环丙沙星、丹诺沙星、恩诺沙星、恩氟沙星和诺氟沙星）和 1×10-6 mol/L（盐酸二氟沙星）。此外，在一定检测范围内，抗生素浓度与 SERS 峰强度之间存在良好的定量关系。实际养殖水样加标测定的结果表明，六种抗生素的回收率在 82.9%至 113.5%之间，相对标准偏差在 1.71%至 7.24%之间。此外，FeO@mTiO@Ag NPs 在辅助水相环境中抗生素的光催化降解方面也取得了令人满意的效果。这为养殖水中抗生素的低浓度检测和高效降解提供了多功能解决方案。
