Zagorul'ko G V, Azhitskiĭ G Iu, Troitskiĭ G V
Vopr Med Khim. 1987 Mar-Apr;33(2):136-8.
A procedure developed involved isoelectrofocusing in borate-polyol system and thin-layer electrophoresis in polyacrylamide gel gradient containing sodium dodecylsulfate of the products of human blood serum albumin obtained after partial hydrolysis. The first dimension isoelectrofocusing in borate-polyol system allowed to separate peptides with close values of pI and to increase the procedure sensitivity. The procedure may be recommended for identification of proteins, for estimation of the protein degradation in hydrolysis as well as for studies of peptide maps of albumin under normal and pathological states.
所开发的一种方法包括在硼酸盐 - 多元醇体系中进行等电聚焦，以及对部分水解后获得的人血清白蛋白产物在含有十二烷基硫酸钠的聚丙烯酰胺凝胶梯度中进行薄层电泳。在硼酸盐 - 多元醇体系中进行的第一维等电聚焦能够分离等电点值相近的肽段，并提高该方法的灵敏度。该方法可推荐用于蛋白质鉴定、水解过程中蛋白质降解的评估以及正常和病理状态下白蛋白肽图的研究。
