Azhitskiĭ G Iu, Zagorul'ko G V, Troitskiĭ G V
Vopr Med Khim. 1987 Jul-Aug;33(4):124-6.
A procedure is described for thin-layer polyacrylamide gel isoelectric focusing involving borate-polyol system with gradient pH 4.0-5.6, where 0.5 M H3BO3-Tris was used as an electrode buffer. Riboflavin, TEMED, Tris-borate buffer and glycerol were used in preparation of gradient pH, which was stable and did not alter during the electrophoresis. Blood serum proteins, particularly blood serum albumin, from patients with diabetes were studied using the isoelectric focusing procedure. After isoelectrofocusing of blood serum albumin from patients with diabetes additional minor acid fractions of the protein were detected at pH 4.3-4.6. The procedure of electrophoresis in gradient pH 4.0-5.6 was highly effective and might be used in biochemical laboratories.
本文描述了一种用于薄层聚丙烯酰胺凝胶等电聚焦的方法，该方法涉及pH值为4.0 - 5.6的硼酸盐 - 多元醇体系，其中使用0.5M的H3BO3 - Tris作为电极缓冲液。在制备pH梯度时使用了核黄素、四甲基乙二胺、Tris - 硼酸盐缓冲液和甘油，该pH梯度稳定，在电泳过程中不会改变。使用等电聚焦方法研究了糖尿病患者的血清蛋白，特别是血清白蛋白。对糖尿病患者的血清白蛋白进行等电聚焦后，在pH 4.3 - 4.6处检测到该蛋白质的额外少量酸性组分。在pH 4.0 - 5.6梯度下的电泳方法非常有效，可用于生化实验室。
