Nikandrov V N, Votiakov V I, Naumovich S A, Vorob'eva G V, Tsymanovich S G
Vopr Med Khim. 1987 Mar-Apr;33(2):54-8.
Streptokinase and heparin developed complex at the ratio 3.2:1, w/w, respectively, and neutral values of pH, which was registered by means of thin-layer chromatography and differential UV-spectroscopy. IR-, differential UV- and CD-spectroscopic methods showed that secondary and tertiary structures of streptokinase were altered in the complex. Streptokinase containing in the complex exhibited the higher thermostability at 50 degrees as compared with native protein. After intravenous administration of the complex into rabbits more distinct alterations were observed in thrombin time, blood clot lysis and in blood plasma fibrinolytic activity as compared with native streptokinase effect.
链激酶和肝素分别以3.2:1（重量比）的比例形成复合物，其pH值呈中性，这是通过薄层色谱法和差示紫外光谱法测定的。红外光谱、差示紫外光谱和圆二色光谱法表明，复合物中链激酶的二级和三级结构发生了改变。与天然蛋白质相比，复合物中的链激酶在50摄氏度时表现出更高的热稳定性。与天然链激酶相比，给兔子静脉注射该复合物后，凝血酶时间、血凝块溶解和血浆纤维蛋白溶解活性出现了更明显的变化。
