College of Animal Science, South China Agricultural University, Guangzhou 510642, China; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.
College of Animal Science, South China Agricultural University, Guangzhou 510642, China; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China; Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
Sci Total Environ. 2022 Dec 10;851(Pt 2):158364. doi: 10.1016/j.scitotenv.2022.158364. Epub 2022 Aug 28.
Advanced wastewater treatment plants are widely used in most large-scale pig farms in southern China. However, the fate of antibiotic resistance genes (ARGs) and their association with bacterial and archaeal communities during advanced wastewater treatment remain unclear. In this study, the profiles of ARGs in typical advanced wastewater treatment plants were surveyed using metagenomic analysis. The results showed that 279- 326 different subtypes of ARGs were detected in raw wastewater, with a total abundance of 5.98 ± 0.48 copies per bacterial cell. During the advanced wastewater treatment, the abundance and number of ARGs were significantly reduced. Microbial communities (bacteria and archaea) contributed the most to the variation in ARG abundance and composition (PCA axis_1), accounting for 10.8 % and 15.7 %, respectively, followed by mobile genetic elements (MGEs) and physicochemical factors. Special attention should be given to potential pathogenic bacteria such as Escherichia, Streptococcus, Enterococcus and Staphylococcus and archaea such as Methanocorpusculum, Candidatus Methanoplasma and Candidatus Methanomethylophilus, which were important potential ARG hosts. Bacterial communities may indirectly affect ARG variation by affecting archaeal communities. These findings indicated that ARG levels in pig farm wastewater can be effectively reduced during advanced treatment and highlighted the important role played by archaea, which should not be ignored.
在中国南方的大多数大型养猪场中，广泛使用高级废水处理厂。然而，抗生素耐药基因（ARGs）在高级废水处理过程中的命运及其与细菌和古菌群落的关系尚不清楚。在本研究中，使用宏基因组分析调查了典型高级废水处理厂中 ARGs 的分布。结果表明，在原废水中检测到 279-326 种不同亚型的 ARGs，其总丰度为每细菌细胞 5.98±0.48 拷贝。在高级废水处理过程中，ARGs 的丰度和数量明显减少。微生物群落（细菌和古菌）对 ARG 丰度和组成的变化贡献最大（PCA 轴_1），分别占 10.8%和 15.7%，其次是移动遗传元件（MGEs）和物理化学因素。应特别注意潜在的病原菌，如 Escherichia、Streptococcus、Enterococcus 和 Staphylococcus，以及古菌，如 Methanocorpusculum、Candidatus Methanoplasma 和 Candidatus Methanomethylophilus，它们是重要的潜在 ARG 宿主。细菌群落可能通过影响古菌群落间接影响 ARG 的变化。这些发现表明，在高级处理过程中可以有效降低养猪场废水中的 ARG 水平，并强调了古菌所起的重要作用，不应忽视。
