Yang Yiwen, Xing Sicheng, Chen Yingxi, Wu Ruiting, Wu Yinbao, Wang Yan, Mi Jiandui, Liao Xindi
Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agriculture University, Guangzhou 510642, China.
Guangdong Laboratory for Lingnan Modern Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agriculture University, Guangzhou 510642, China; Key Laboratory of Tropical Agricultural Environment, Ministry of Agriculture, South China Agricultural University, Guangzhou 510642, China.
J Hazard Mater. 2021 Feb 15;404(Pt B):124149. doi: 10.1016/j.jhazmat.2020.124149. Epub 2020 Oct 1.
In this study, the profiles of bacteria/phage-comediated antibiotic resistance genes (b/pARGs) were monitored in water samples collected from 45 pig farm wastewater treatment plants (WWTPs) in seven different regions of China. We found that 8 major types and 112 subtypes of b/pARGs were detected in all the water samples, and the detected number ranged from 53 to 92. The absolute abundances of bARGs and pARGs in the influent were as high as 10 copies/mL and 10 copies/mL, respectively. Anaerobic anoxic/oxic (AAO) and anaerobic short-cut nitrification/denitrification (ASND) treatment plants can effectively reduce the absolute abundance and amount of b/pARGs. Anaerobic treatment plants cannot reduce the absolute abundance of pARGs, and even increase the amount of pARGs. Mobile genetic elements (MGEs), bacterial communities and environmental factors were important factors impacting the b/pARG profile. Among these factors, the bacterial community was the major driver that impacted the bARG profile, while bacterial community and MGEs were the major codrivers impacting the pARG profile. This study was the first to investigate the profiles of b/pARGs in pig farm WWTPs in China on such a large scale, providing a reference for the prevention and control of ARG pollution in agricultural environments.
在本研究中，对从中国七个不同地区的45个养猪场废水处理厂（WWTPs）采集的水样中的细菌/噬菌体介导的抗生素抗性基因（b/pARGs）谱进行了监测。我们发现，在所有水样中检测到8种主要类型和112个亚型的b/pARGs，检测数量范围为53至92个。进水bARGs和pARGs的绝对丰度分别高达10拷贝/毫升和10拷贝/毫升。厌氧-缺氧/好氧（AAO）和厌氧短程硝化/反硝化（ASND）处理厂可以有效降低b/pARGs的绝对丰度和数量。厌氧处理厂不能降低pARGs的绝对丰度，甚至会增加pARGs的数量。移动遗传元件（MGEs）、细菌群落和环境因素是影响b/pARG谱的重要因素。在这些因素中，细菌群落是影响bARG谱的主要驱动因素，而细菌群落和MGEs是影响pARG谱的主要共同驱动因素。本研究首次如此大规模地调查了中国养猪场WWTPs中的b/pARGs谱，为农业环境中ARG污染的防控提供了参考。
