再充气对体内过期储存兔红细胞存活率的影响。

The effects of reinflation on the survival of time-expired stored rabbit erythrocytes in vivo.

作者信息

Turner S, Williams A R, Rees J M

出版信息

Vox Sang. 1987;52(3):186-90. doi: 10.1111/j.1423-0410.1987.tb03024.x.

DOI:10.1111/j.1423-0410.1987.tb03024.x

Abstract

Rabbit blood cells stored for prolonged periods in citrate phosphate dextrose with adenine (CPDA-1) at +4 degrees C show the same decreased volume, increased cell density and decreased filterability as human cells stored under similar conditions. As with human erythrocytes, the stored rabbit cells had to be incubated with autologous fresh plasma for 24 h at 37 degrees C before these changes could be observed (this incubation process apparently mimics the effects of reinfusion). These shrunken stored rabbit cells could also be reinflated using nystatin, so that their mean cell volume, mean corpuscular haemoglobin concentration, average cell densities and filterabilities were restored to normal values. This in vitro reinflation of the stored cells significantly prolonged their in vivo survival rate as determined by radiochromium labelling studies. This reinflation technique may therefore have an application in the prolongation of the useful storage life of human blood.

摘要

在+4摄氏度下于含腺嘌呤的枸橼酸盐磷酸盐葡萄糖（CPDA-1）中长时间储存的兔血细胞，与在类似条件下储存的人类细胞一样，呈现出相同的体积减小、细胞密度增加和滤过性降低的情况。与人类红细胞一样，储存的兔细胞在这些变化能够被观察到之前，必须在37摄氏度下与自体新鲜血浆孵育24小时（这种孵育过程显然模拟了再输注的效果）。这些皱缩的储存兔细胞也可以使用制霉菌素使其再膨胀，从而使它们的平均细胞体积、平均红细胞血红蛋白浓度、平均细胞密度和滤过性恢复到正常值。通过放射性铬标记研究确定，储存细胞的这种体外再膨胀显著延长了它们在体内的存活率。因此，这种再膨胀技术可能在延长人类血液的有效储存寿命方面具有应用价值。