Brydak L, Liubotseva O V, Iakhno M A, Zakstel'skaia L Ia
Vopr Virusol. 1987 Mar-Apr;32(2):156-9.
A method for isolation of neuraminidase using pronase has been developed. Purified preparations have been recovered from two influenza B virus strains, B/Mass/3/66 and B/USSR/14/80. Physico-chemical properties of neuraminidases were compared; optimal conditions for the determination of neuraminidase activity of these strains were determined. Strains differences in thermostability, optimal pH, specificity to substrates were established. Neuraminidases, isolated or present in virions, were shown to be identical in enzymatic characteristics but to differ in thermostability.
已开发出一种使用链霉蛋白酶分离神经氨酸酶的方法。已从两种乙型流感病毒株B/Mass/3/66和B/USSR/14/80中获得了纯化制剂。比较了神经氨酸酶的物理化学性质；确定了测定这些毒株神经氨酸酶活性的最佳条件。确定了毒株在热稳定性、最佳pH值、对底物的特异性方面的差异。结果表明，分离的或存在于病毒粒子中的神经氨酸酶在酶学特性上是相同的，但在热稳定性上有所不同。
