Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, 5166616471, Iran.
Institute of Animal Science, Physiology Unit, University of Bonn, 53115, Bonn, Germany.
Sci Rep. 2022 Aug 30;12(1):14748. doi: 10.1038/s41598-022-18956-5.
A growing body of evidence shows that microRNA (miRNA), play important roles in regulating adipose tissue (AT) physiology and function. The objective was to characterize the AT miRNA profile in over-conditioned (HBCS, n = 19) versus moderate-conditioned (MBCS, n = 19) periparturient dairy cows. Tail-head subcutaneous AT biopsied on d -49 and 21 relative to parturition were used for miRNA sequencing. The miR-486 was the most significant miRNA among the upregulated miRNA on d -49, which might be related to more pronounced changes in lipogenesis and altered insulin sensitivity in AT of HBCS cows at dry-off. Comparing HBCS to MBCS on d 21, 23 miRNA were downregulated and 20 were upregulated. The predicted targets of upregulated differentially expressed (DE)-miRNA on d 21 were enriched in different pathways, including pathways related to lysosomes and peroxisomes. The predicted targets of downregulated DE-miRNA on d 21 were enriched in various pathways, including epidermal growth factor receptor, insulin resistance, hypoxia-inducible factor 1 signaling pathway, and autophagy. The results showed that over-conditioning was associated with changes in SCAT miRNA profile mainly on d 21, of which most were downregulated. The enriched pathways may participate in over-conditioning-associated metabolic challenges during early lactation.
越来越多的证据表明，微小 RNA（miRNA）在调节脂肪组织（AT）生理和功能方面发挥着重要作用。本研究旨在描述过度饲养（HBCS，n=19）与适度饲养（MBCS，n=19）围产期奶牛的尾部皮下 AT 组织 miRNA 图谱。在分娩前 49 天和 21 天分别对尾头皮下 AT 进行 miRNA 测序。miR-486 是 d-49 上调 miRNA 中最显著的 miRNA，可能与干奶期 HBCS 奶牛 AT 中脂肪生成和胰岛素敏感性变化更明显有关。与 MBCS 相比，HBCS 在 d 21 时有 23 个 miRNA 下调，20 个 miRNA 上调。d 21 上调差异表达（DE）miRNA 的预测靶标富集在不同的途径中，包括与溶酶体和过氧化物酶体相关的途径。d 21 下调 DE-miRNA 的预测靶标富集在各种途径中，包括表皮生长因子受体、胰岛素抵抗、缺氧诱导因子 1 信号通路和自噬。结果表明，过度饲养与 d 21 时 SCAT miRNA 谱的变化有关，其中大多数 miRNA 下调。富集的途径可能参与了早期泌乳期间与过度饲养相关的代谢挑战。
