Department of Cardiology, University Hospital, Medical Clinic II, University Heart Center Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany.
Department of Cardiology, University Hospital, Medical Clinic II, University Heart Center Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany.
STAR Protoc. 2022 Aug 19;3(3):101645. doi: 10.1016/j.xpro.2022.101645. eCollection 2022 Sep 16.
Here, we describe an approach to visualize CD11c cells in atherosclerosis. In particular, we use a protocol for X-Gal staining of immune cells within atherosclerotic plaques, which can be used as an alternative to analyze plaque composition and cell-specific molecules in atherogenesis. LacZ knockin mice have to be bred to mice carrying the CD11c recombinase-both brought onto an ApoE background-to be able to visualize this cell type of interest in the plaques by X-Gal staining. With this approach, different immune cells in atherogenesis can be examined. For complete details on the use and execution of this protocol, please refer to Sauter et al. (2021).
在这里，我们描述了一种可视化动脉粥样硬化中 CD11c 细胞的方法。具体来说，我们使用了一种用于动脉粥样硬化斑块内免疫细胞 X-Gal 染色的方案，该方案可用于替代分析动脉粥样发生过程中斑块组成和细胞特异性分子。为了能够通过 X-Gal 染色来可视化斑块中的这种感兴趣的细胞类型，必须将 LacZ 基因敲入小鼠与携带 CD11c 重组酶的小鼠杂交——这两种小鼠都被带到 ApoE 背景下。通过这种方法，可以检查动脉粥样发生过程中的不同免疫细胞。有关该方案使用和执行的完整详细信息，请参阅 Sauter 等人（2021 年）。
