Department of Cardiology, University Hospital, Medical Clinic II, University Heart Center Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany.
Department of Cardiology, University Hospital, Medical Clinic II, University Heart Center Luebeck, Ratzeburger Allee 160, 23538 Luebeck, Germany.
STAR Protoc. 2022 Sep 16;3(3):101664. doi: 10.1016/j.xpro.2022.101664. Epub 2022 Sep 8.
Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c cells and CD11c-derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating cells in culture dishes, treating them with differentiation factors, and collecting cells after removal of undesirable populations. This protocol can be adapted for CD11c cells in different contexts, thus, serving as models for different diseases and to analyze cell-specific molecules. For complete details on the use and execution of this protocol, please refer to Sauter et al. (2021).
不同类型的免疫细胞参与动脉粥样硬化的发生，可能发挥抗动脉粥样硬化或促动脉粥样硬化作用。在这里，我们描述了一种分析动脉粥样硬化中 CD11c 细胞和 CD11c 衍生的 ApoE 的体外方法。主要步骤包括收获小鼠骨髓、在培养皿中铺板细胞、用分化因子处理细胞，以及在去除不需要的细胞群后收集细胞。该方案可以适应不同背景下的 CD11c 细胞，因此可作为不同疾病的模型，并分析细胞特异性分子。有关该方案使用和执行的完整详细信息，请参阅 Sauter 等人（2021 年）。
