从初始多能干细胞中直接衍生人滋养层干细胞的方案。
Protocol to derive human trophoblast stem cells directly from primed pluripotent stem cells.
机构信息
Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON M5T3H7, Canada; Key Laboratory of Functional Proteomics of Guangdong Province, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China; The First Clinical Medical School, Southern Medical University, Guangzhou 510515, China.
Placenta Research Group, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
出版信息
STAR Protoc. 2022 Aug 18;3(3):101638. doi: 10.1016/j.xpro.2022.101638. eCollection 2022 Sep 16.
Human trophoblast stem cells (hTSCs) are useful for studying human placenta development and diseases, but primed human pluripotent stem cells (hPSCs) routinely cultured in most laboratories do not support hTSC derivation. Here, we present a protocol to derive hTSCs directly from primed hPSCs. This approach, containing two strategies either with or without bone morphogenetic protein 4 (BMP4), provides a simple and accessible tool for deriving hTSCs to study placenta development and disease modeling without ethical limitations or reprogramming process. For complete details on the use and execution of this protocol, please refer to Wei et al. (2021).
人滋养层干细胞（hTSCs）可用于研究人类胎盘发育和疾病，但大多数实验室常规培养的初始人多能干细胞（hPSCs）并不支持 hTSC 的衍生。在此，我们提出了一种直接从初始 hPSCs 中衍生 hTSCs 的方案。该方法包含两种策略，无论是否使用骨形态发生蛋白 4（BMP4），都为研究胎盘发育和疾病建模提供了一种简单且易于使用的工具，而没有伦理限制或重编程过程。如需详细了解本方案的使用和执行，请参考 Wei 等人（2021 年）的研究。
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