Oğuz Osman, Serin Huriye, Hocaoglu Fatma Sinem
Istanbul Education and Research Hospital, Department of Medical Biochemistry, Istanbul, Turkey.
Düzen Lab, Department of Clinical Biochemistry, Istanbul,Turkey.
J Med Biochem. 2022 Jul 29;41(3):335-340. doi: 10.5937/jomb0-33981.
Alkaline phosphatase (ALP) enzymes are widely used as signal amplifiers in immunoenzymatic methods. Conditions that cause ALP elevations, such as bone or liver diseases, can cause interference in immunoenzymatic methods. We aimed to examine ALP's effect on immunoenzymatic assay by adding isolated pure ALP to the prepared serum pool.
We prepared a serum pool and divided it into 4 groups. By adding isolated pure ALP at different concentrations to each group, we obtained sample groups containing ALP enzyme at concentrations of 85 U/L, 340 U/L, 870 U/L, and 1570 U/L. 20-repetition of bhCG, ferritin, FT4, TSH, troponin I, and Vit B12 tests were performed in each group. The coefficient of variation, bias, and total error was calculated. All groups were compared by using the Friedman test for paired samples.
After ALP addition, the calculated total error values of FT4, bhCG and troponin I tests were above the acceptable error limits. There were statistically significant differences in bhCG, FT4, troponin I, and Vit B12 tests compared to the baseline ALP level (P<0.0125).
Isolated ALP elevations can be a source of interference for immunoenzymatic methods.
碱性磷酸酶（ALP）酶在免疫酶法中被广泛用作信号放大器。导致ALP升高的情况，如骨骼或肝脏疾病，可能会干扰免疫酶法。我们旨在通过向制备的血清池中添加分离出的纯ALP来研究ALP对免疫酶测定的影响。
我们制备了一个血清池并将其分为4组。通过向每组添加不同浓度的分离出的纯ALP，我们获得了ALP酶浓度分别为85 U/L、340 U/L、870 U/L和1570 U/L的样本组。对每组进行20次β-人绒毛膜促性腺激素（bhCG）、铁蛋白、游离甲状腺素（FT4）、促甲状腺激素（TSH）、肌钙蛋白I和维生素B12测试。计算变异系数、偏差和总误差。使用配对样本的弗里德曼检验对所有组进行比较。
添加ALP后，FT4、bhCG和肌钙蛋白I测试的计算总误差值高于可接受误差限。与基线ALP水平相比，bhCG、FT4、肌钙蛋白I和维生素B12测试存在统计学显著差异（P<0.0125）。
分离出的ALP升高可能是免疫酶法干扰的一个来源。
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