Rughwani Roshan R, Cholan Priyanka K, Victor Dhayanand John, Ilango Paavai, Cherian Sanjay M, Rughwani Rajkumar N, Tadepalli Anupama
Department of Periodontics, SRM Dental College, Ramapuram, Chennai, 600 089, India.
Dental Department, Frontier Lifeline Hospital, Chennai, 600 037, India.
J Oral Biol Craniofac Res. 2022 Sep-Oct;12(5):633-638. doi: 10.1016/j.jobcr.2022.08.013. Epub 2022 Aug 17.
and Purpose: The aim of this study was to assess, compare and correlate the ability of PCSK9 and IL6 as potential common serum and salivary biomarkers for stage III/IV periodontitis and the risk of developing atherosclerotic cardiovascular diseases.
76 patients were alloted into 4 groups consisting of subjects with clinically healthy periodontium and stage III/IV periodontitis with and without ACVD. Clinical parameters - PD, CAL, number of teeth, PI, mSBI, TC, Tg, HDL and LDL were recorded. Serum and saliva samples were obtained and subjected to ELISA for quantifying the biomarker levels.
The level of these biomarkers was found to be the lowest (IL6 Serum: 21.92 ± 14.54, IL6 Saliva: 12.34 ± 7.72, PCSK9 Serum: 178.82 ± 35.07, PCSK9 Saliva: 80.82 ± 25.43) in group I and highest in group IV (IL6 Serum: 73.4 ± 24.86, IL6 Saliva: 37.66 ± 15.77, PCSK9 Serum: 346.54 ± 45.11, PCSK9 Saliva: 157.72 ± 43.28). The clinical parameters PI, mSBI, PPD, CAL, total number of teeth, TC, HDL and LDL showed a significant correlation with biomarkers PCSK9 and IL6.
This study features the elevation of serum and salivary PCSK9 and IL6 in periodontitis and ACVD. A triad of serum and salivary PCSK9 and IL6 along with the clinical markers of periodontal disease can relatively predict the future risk of developing ACVD.
本研究旨在评估、比较并关联前蛋白转化酶枯草溶菌素9（PCSK9）和白细胞介素6（IL6）作为III/IV期牙周炎潜在的常见血清和唾液生物标志物的能力，以及发生动脉粥样硬化性心血管疾病的风险。
76名患者被分为4组，包括临床牙周健康者以及患有和未患有动脉粥样硬化性心血管疾病（ACVD）的III/IV期牙周炎患者。记录临床参数——牙周袋深度（PD）、临床附着丧失（CAL）、牙齿数量、菌斑指数（PI）、改良龈沟出血指数（mSBI）、总胆固醇（TC）、甘油三酯（Tg）、高密度脂蛋白（HDL）和低密度脂蛋白（LDL）。采集血清和唾液样本，通过酶联免疫吸附测定（ELISA）法对生物标志物水平进行定量分析。
发现这些生物标志物的水平在I组中最低（IL6血清：21.92±14.54，IL6唾液：12.34±7.72，PCSK9血清：178.82±35.07，PCSK9唾液：80.82±25.43），在IV组中最高（IL6血清：73.4±24.86，IL6唾液：37.66±15.77，PCSK9血清：346.54±45.11，PCSK9唾液：157.72±43.28）。临床参数PI、mSBI、PD、CAL、牙齿总数、TC、HDL和LDL与生物标志物PCSK9和IL6显示出显著相关性。
本研究表明牙周炎和ACVD患者血清和唾液中PCSK9和IL6水平升高。血清和唾液中的PCSK9和IL6三联体以及牙周疾病的临床标志物可以相对预测未来发生ACVD的风险。
