Department of Pediatrics, Xuzhou First People's Hospital, Xuzhou, Jiangsu, China.
Contrast Media Mol Imaging. 2022 Aug 8;2022:2208636. doi: 10.1155/2022/2208636. eCollection 2022.
This study aimed to study the application value of a new multichannel sensor in pathogen detection and drug resistance analysis of neonatal pneumonia. 180 newborns with infectious pneumonia were selected, and a new multichannel piezoelectric sensor was constructed. The traditional Kirby-Bauer (K-B) method and the piezoelectric sensor were adopted to detect the pathogens and drug resistance in newborn samples, respectively. The results showed that the sensitivity and specificity under the K-B method (99.58% and 99.32%) and the multichannel piezoelectric sensor (99.43% and 94.29%) were not statistically different ( > 0.05). The detection time (17.25 h) of the K-B method was significantly longer than that (7.43 h) of the multichannel piezoelectric sensor ( < 0.05). From the results of pathogen detection, it was found that accounted for a relatively high proportion of 25.1%, followed by and of 13.4% and 12.33%, respectively. The resistance rate of the to vancomycin and rifampicin was as high as 100% and that to gentamicin, ciprofloxacin, and erythromycin reached more than 50%. In short, the new multichannel piezoelectric sensor had the high sensitivity and specificity for the pathogens' detection of neonatal pneumonia, and it required a shorter time. The pathogens were mostly Gram-negative bacteria, followed by Gram-positive bacteria and fungi. , , and were the main ones. The neonatal pneumonia pathogens had also strong drug resistance against vancomycin, rifampicin, chloramphenicol, meropenem, amikacin sulfate, chloramphenicol, and many other antibacterial drugs.
本研究旨在探讨新型多通道传感器在新生儿肺炎病原体检测及耐药性分析中的应用价值。选取 180 例感染性肺炎新生儿，构建新型多通道压电传感器，分别采用传统 Kirby-Bauer（K-B）法和压电传感器检测新生儿样本中的病原体及其耐药性。结果显示，K-B 法的灵敏度和特异度分别为 99.58%和 99.32%，多通道压电传感器分别为 99.43%和 94.29%，差异均无统计学意义（>0.05）。K-B 法的检测时间（17.25 h）显著长于多通道压电传感器（7.43 h）（<0.05）。从病原体检测结果来看，占比较高的分别为 25.1%，其次为 、 ，分别为 13.4%、12.33%。对万古霉素和利福平的耐药率高达 100%，对庆大霉素、环丙沙星和红霉素的耐药率均超过 50%。总之，新型多通道压电传感器对新生儿肺炎病原体检测具有较高的灵敏度和特异性，且所需时间更短。病原体多为革兰阴性菌，其次为革兰阳性菌和真菌。、、是主要的病原体。新生儿肺炎病原体对万古霉素、利福平、氯霉素、美罗培南、硫酸阿米卡星、氯霉素等多种抗菌药物也具有较强的耐药性。
