Ikeuchi Aoi, Kondoh Daisuke, Halajian Ali, Ichikawa-Seki Madoka
Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka, 020-8550, Japan.
Laboratory of Veterinary Anatomy, Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada-cho Nishi, Obihiro, 080-8555, Hokkaido, Japan.
Int J Parasitol Parasites Wildl. 2022 Aug 10;19:38-43. doi: 10.1016/j.ijppaw.2022.08.003. eCollection 2022 Dec.
Paramphistomes, commonly known as rumen flukes, are digenean parasites that infect ruminants. Accurate morphological identification of paramphistome species is challenging and often neglected. For instance, it requires sagittal midline sections of adult flukes, which are difficult to prepare. Therefore, the majority of the genetic information on paramphistomes found in the International Nucleotide Sequence Database is not supported by morphological descriptions, and the DNA barcodes of paramphistome species remain unreliable. In the present study, both morphological and molecular characterizations were simultaneously performed to ensure the reliability of the DNA information for the paramphistome species (Näsmark, 1937). The morphological characteristics of the sagittal and horizontal sections of adult flukes from a black wildebeest () and a waterbuck () in South Africa were identical to those previously described for Additionally, this study represents a new host record of the species from . All sequences of the internal transcribed spacer 2 region of ribosomal DNA were 100% identical among the 18 flukes analyzed in the present study. A single nucleotide mutation was observed between in this study and detected in domestic ruminants in Kenya.
双口吸虫，通常被称为瘤胃吸虫，是感染反刍动物的复殖吸虫寄生虫。准确地从形态学上鉴定双口吸虫物种具有挑战性，且常常被忽视。例如，这需要成年吸虫的矢状中线切片，而这种切片很难制备。因此，国际核苷酸序列数据库中发现的关于双口吸虫的大多数遗传信息都没有形态学描述的支持，双口吸虫物种的DNA条形码仍然不可靠。在本研究中，同时进行了形态学和分子特征分析，以确保双口吸虫物种（Näsmark，1937）DNA信息的可靠性。来自南非一头黑角马（）和一头水羚（）的成年吸虫矢状和水平切片的形态特征与先前描述的该物种的特征相同。此外，本研究代表了该物种来自新的宿主记录。在本研究分析的18条吸虫中，核糖体DNA内转录间隔区2区域的所有序列100%相同。在本研究中的该物种与在肯尼亚家养反刍动物中检测到的该物种之间观察到一个单核苷酸突变。
