Marmolejo-Guzmán Linda Yacsiri G, Hernández-Mena David Iván G, Castellanos-Martínez Sheila, Aguirre-Macedo M Leopoldina
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional Unidad Mérida, Antigua Carretera a Progreso, Km. 6 S/N, CORDEMEX, CP. 97310, Mérida, Yucatán, Mexico.
Instituto de Investigaciones Oceanológicas, Universidad Autónoma de Baja California, Carretera Transpeninsular Ensenada-Tijuana 3917, Fraccionamiento Playitas, 22860, Ensenada, Baja, CA, Mexico.
Int J Parasitol Parasites Wildl. 2022 Aug 9;19:44-55. doi: 10.1016/j.ijppaw.2022.08.001. eCollection 2022 Dec.
is an endemic species of the Yucatan Peninsula. This species sustains the octopus' fishery in the region and is the only cephalopod cultured in Mexico. It is known that harbor a large richness and abundance of metacestodes that have been tentatively identified by light microscopy alone. Since the larval stages of some orders of marine cestodes lack the taxonomic characteristics shown by the adult stages and on which cestode taxonomy is based, identification down to the species level is often unattainable. Hence, the goal of this study was to characterize the parasites, for the first time, at morphological and molecular levels. A total of 60 octopuses were collected from September to December 2017 from four fishery landing ports in Yucatán: Sisal, Progreso, Dzilam de Bravo, and Rio Lagartos (15 hosts per locality). Morphology of metacestodes was characterized by light and Scanning Electron Microscopy (SEM), while the genes 18S and 28S rDNA were sequenced for molecular characterization. Based on phenotypic characters and molecular data, seven taxa of metacestodes were identified, four of them belonging of order Trypanorhyncha: sp., , sp., sp. 1; and the three remaining taxa belonging to the order Onchoproteocephalidea: sp., sp., and sp. This work provides, for the first time, molecular support to the morphological characterization of metacestodes recorded in
是尤卡坦半岛的特有物种。该物种支撑着该地区的章鱼渔业，并且是墨西哥唯一养殖的头足类动物。已知其体内寄生着大量丰富的拟囊尾蚴，此前仅通过光学显微镜初步鉴定过。由于某些海洋绦虫目幼虫阶段缺乏成虫阶段所具有的、作为绦虫分类学基础的分类特征，因此往往无法鉴定到物种水平。因此，本研究的目标是首次在形态学和分子水平上对这些寄生虫进行特征描述。2017年9月至12月，从尤卡坦的四个渔业上岸港口：锡萨尔、普罗格雷索、齐拉姆·德·布拉沃和里奥拉加托斯共采集了60只章鱼（每个地点15只宿主）。通过光学显微镜和扫描电子显微镜（SEM）对拟囊尾蚴的形态进行了特征描述，同时对18S和28S rDNA基因进行测序以进行分子特征分析。基于表型特征和分子数据，鉴定出了7个拟囊尾蚴分类单元，其中4个属于锥吻目：[具体物种1]种、[具体物种2]种、[具体物种3]种、锥吻目未定种1；其余3个分类单元属于圆叶目：[具体物种4]种、[具体物种5]种和[具体物种6]种。这项工作首次为记录在[相关内容]中的拟囊尾蚴的形态特征提供了分子支持。
