Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland.
Chemistry. 2022 Nov 25;28(66):e202202114. doi: 10.1002/chem.202202114. Epub 2022 Oct 1.
In this contribution we report the high-resolution NMR structure of a recently identified lanthanide-binding aptamer (LnA). We demonstrate that the rigid lanthanide binding by LnA allows for the measurement of anisotropic paramagnetic NMR restraints which to date remain largely inaccessible for nucleic acids. One type of such restraints - pseudocontact shifts (PCS) induced by four different paramagnetic lanthanides - was extensively used throughout the current structure determination study and the measured PCS turned out to be exceptionally well reproduced by the final aptamer structure. This finding opens the perspective for a broader application of paramagnetic effects in NMR studies of nucleic acids through the transplantation of the binding site found in LnA into other DNA/RNA systems.
在本研究中，我们报道了一种新鉴定的镧系元素结合适体（LnA）的高分辨率 NMR 结构。我们证明，LnA 对镧系元素的刚性结合允许测量各向异性顺磁 NMR 约束，迄今为止，这些约束在很大程度上无法用于核酸。这些约束中的一种类型 - 由四种不同的顺磁镧系元素引起的赝接触位移（PCS） - 在当前的结构确定研究中得到了广泛应用，并且所测量的 PCS 被最终的适体结构异常良好地再现。这一发现为通过将在 LnA 中发现的结合位点移植到其他 DNA/RNA 系统中，在核酸的 NMR 研究中更广泛地应用顺磁效应开辟了前景。
