Department of Pharmacy, Al-Zaytoonah University of Jordan, Amman 11733, Jordan.
Department of Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan.
Endocr Metab Immune Disord Drug Targets. 2023;23(5):681-691. doi: 10.2174/1871530322666220827143530.
Diabetes mellitus is a long-term disorder with high prevalence globally. It can be classified into two types: Type 1 diabetes and Type 2 diabetes mellitus. Diabetes mellitus is considered a multifactorial disorder in which genetic factors such as cytokines play a major role. Cytokines play a role in immune modulation and are associated with the development of diabetes mellitus. Single nucleotide polymorphisms in cytokines were studied extensively in different populations to determine their association with a predisposition to diabetes mellitus.
The aim of this study was to estimate the frequency of single nucleotide polymorphisms in the cytokine genes TNF-α, TGF-β, IL-6, IL-10, and IFN-γ in 102 Jordanian diabetes mellitus patients in comparison to 50 controls and their association to diabetes mellitus susceptibility.
Analysis was performed using the highly specific polymerase chain reaction-sequence specific primers methodology.
Our findings showed that the IL-10-1082 G/G genotype (P = 0.02) and the TGF-β1 codon 25G allele (P < 0.01) may be considered risk factors for type 2 diabetes mellitus. In addition, the IFN-γ-874A allele (P = 0.04) seems to increase the predisposition to type 1 diabetes.
Our study showed that the IL-10-1082 G/G genotype and TGF-β1 codon 25G allele are associated with type 2 diabetes mellitus while the IFN- γ -874A allele is associated with type 1 diabetes. Our findings may help in the early detection of diabetes mellitus, which would in turn help in undergoing the needed preventative measures to delay the onset of diabetes mellitus.
糖尿病是一种全球高发的慢性疾病，可分为 1 型糖尿病和 2 型糖尿病。糖尿病被认为是一种多因素疾病，其中细胞因子等遗传因素起着重要作用。细胞因子在免疫调节中发挥作用，并与糖尿病的发生有关。细胞因子的单核苷酸多态性在不同人群中进行了广泛研究，以确定它们与糖尿病易感性的关系。
本研究旨在估计 102 例约旦糖尿病患者和 50 例对照者细胞因子基因 TNF-α、TGF-β、IL-6、IL-10 和 IFN-γ 中单核苷酸多态性的频率，并分析其与糖尿病易感性的关系。
采用聚合酶链反应-序列特异性引物法进行分析。
我们的研究结果表明，IL-10-1082 G/G 基因型（P = 0.02）和 TGF-β1 密码子 25G 等位基因（P < 0.01）可能是 2 型糖尿病的危险因素。此外，IFN-γ-874A 等位基因（P = 0.04）似乎增加了 1 型糖尿病的易感性。
本研究表明，IL-10-1082 G/G 基因型和 TGF-β1 密码子 25G 等位基因与 2 型糖尿病有关，而 IFN-γ-874A 等位基因与 1 型糖尿病有关。我们的发现可能有助于早期发现糖尿病，从而有助于采取必要的预防措施来延迟糖尿病的发病。
