School of Medicine, Southern University of Science and Technology, No 1088 Xueyuan Blvd, Xili, Nanshan District, Shenzhen, Guangdong, 518055, China.
Department of Pediatric Neurology, Shenzhen Children's Hospital, 7019 Yitian Road, Shenzhen, 518038, China.
J Mater Chem B. 2022 Sep 21;10(36):7045-7051. doi: 10.1039/d2tb01441f.
Lysosomal pH is an important indicator for the physiological state of eukaryotic cells. The real-time detection of intracellular lysosomal pH is critical for understanding and studying many physiological and pathological processes of cells. Herein, we designed and synthesized a series of novel pH sensors, namely W1, W2 and W3. By comparing the spectroscopic properties of the three molecules and their ability to target lysosomes in living cells, a specific probe W1 was selected for the quantitative analysis of lysosomal pH changes in live cells. W1 shows a fast, sensitive and highly selective red fluorescence response to an acidic pH value. The p value of W1 is 5.84, and the fluorescence intensity ratios of / under acidic conditions show a good linear relationship with the pH value. In addition, W1 shows a 100-fold difference in fluorescence from an extracellular environment to an intracellular environment, allowing it to be used as a "wash free" staining probe to visualize the pH change of lysosomes. W1 was further applied to detect the changes of lysosomal pH during apoptosis and mitophagy. Thus, W1 is expected to be a potentially useful tool for monitoring the changes of lysosomal pH in cell-related physiological or pathological states.
溶酶体 pH 值是真核细胞生理状态的一个重要指标。实时检测细胞内溶酶体 pH 值对于理解和研究细胞的许多生理和病理过程至关重要。在此，我们设计并合成了一系列新型 pH 传感器，即 W1、W2 和 W3。通过比较这三种分子的光谱性质及其在活细胞中靶向溶酶体的能力，选择了一个特定的探针 W1 用于定量分析活细胞中溶酶体 pH 值的变化。W1 对酸性 pH 值表现出快速、灵敏和高度选择性的红色荧光响应。W1 的 p 值为 5.84，在酸性条件下的荧光强度比值与 pH 值呈良好的线性关系。此外，W1 在细胞外环境和细胞内环境中的荧光差异有 100 倍，这使其可用作“免洗”染色探针来可视化溶酶体 pH 值的变化。W1 进一步被用于检测细胞凋亡和线粒体自噬过程中溶酶体 pH 值的变化。因此，W1 有望成为监测细胞相关生理或病理状态下溶酶体 pH 值变化的一种潜在有用工具。
