酶联免疫吸附测定法（ELISA）用于足菌肿血清学诊断的初步研究。

Preliminary investigation of the use of the enzyme linked immunosorbent assay (ELISA) in the serodiagnosis of mycetoma.

作者信息

McLaren M L, Mahgoub E S, Georgakopoulos E

出版信息

Sabouraudia. 1978 Sep;16(3):225-8. doi: 10.1080/00362177885380301.

Abstract

Antibody levels in a small group of Sudanese patients with clinically diagnosed mycetoma, and control groups were measured by countercurrent immunoelectrophoresis (CIE) and enzyme linked immunosorbent assay (ELISA). Antigens were prepared from the following organisms: Streptomyces somaliensis, Actinomadura madurae, A. pelletieri, Nocardia brasiliensis and Madurella mycetomi. Positive reactions were obtained in clinical cases against the homologous antigens in both CIE and ELISA; all heterologous and control sera negative in CIE but produced some reaction particularly against N. brasiliensis and M. mycetomi antigens in ELISA.

摘要

通过对流免疫电泳（CIE）和酶联免疫吸附测定（ELISA）检测了一小群临床诊断为足菌肿的苏丹患者以及对照组的抗体水平。抗原由以下微生物制备：索马里链霉菌、马杜拉放线菌、佩氏放线菌、巴西诺卡菌和马杜拉霉样真菌。临床病例在CIE和ELISA中对同源抗原均呈阳性反应；所有异源血清和对照血清在CIE中呈阴性，但在ELISA中产生了一些反应，尤其是对巴西诺卡菌和马杜拉霉样真菌抗原的反应。