Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P.R. China.
Department of Orthopedic Surgery, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China.
Langmuir. 2022 Sep 13;38(36):11130-11135. doi: 10.1021/acs.langmuir.2c02102. Epub 2022 Aug 31.
MicroRNAs (miRNAs) are a family of endogenous noncoding RNAs with the functions of gene regulation, which serve as promising markers for a range of diseases such as diabetic foot ulcers, cancers, etc. In this work, we engineered a roll-to-roll DNA nanomachine for highly sensitive electrochemical detection of miRNA. A dumbbell-structured DNA probe could be transitioned to be wheel-structured conformation upon target recognition, which rolls around track strands on the surface of gold nanoparticles (AuNPs) in the presence of nicking endonuclease. The resulting single strands on AuNPs are activated for the second round of rolling at the DNA-modified electrode interface, leading to the variation of electrochemical responses. The roll-to-roll amplification behavior allows a wide detection range with a limit of detection as low as 10 aM. The practicability is also demonstrated by the application in human serum samples with satisfactory results. It is expected that the proposed electrochemical method offers a new paradigm to develop miRNA assays based on DNA nanotechnology.
微小 RNA（miRNA）是一类具有基因调控功能的内源性非编码 RNA，可作为多种疾病（如糖尿病足溃疡、癌症等）的有前途的标志物。在这项工作中，我们设计了一种卷对卷 DNA 纳米机器，用于 miRNA 的高灵敏度电化学检测。哑铃结构的 DNA 探针在靶标识别后可以转变为轮状构象，在切口内切酶存在下，在金纳米粒子（AuNPs）表面的轨道链上滚动。在 DNA 修饰电极界面上，AuNPs 上生成的单链被激活以进行第二轮滚动，从而导致电化学响应的变化。滚到滚的放大行为允许具有低至 10 aM 的检测限的宽检测范围。通过在人血清样本中的应用也证明了其实用性，结果令人满意。预计所提出的电化学方法为基于 DNA 纳米技术的 miRNA 分析提供了一个新的范例。
