Siemion I Z, Stefanowicz P, Jankowski A
Acta Biochim Pol. 1987;34(1):11-20.
It was found that N-acetyl-DL-(p-N',N'-dimethylamino)phenylalanine, in the form of azlactone, can be introduced into a peptide or protein molecule as a new convenient fluorescent marker. A fluorophore of similar properties: N-acetyl-(p-amino)phenylalanine can be introduced into a peptide chain by the reaction with the azlactone of N-acetyl-(p-nitro)phenylalanine followed by reduction of nitro group to amino group. This method, however, cannot be applied to proteins.
已发现，氮杂环丙烷形式的N-乙酰-DL-（对-N'，N'-二甲基氨基）苯丙氨酸可作为一种新型便捷的荧光标记引入肽或蛋白质分子中。具有类似性质的荧光团：N-乙酰-（对氨基）苯丙氨酸可通过与N-乙酰-（对硝基）苯丙氨酸的氮杂环丙烷反应，随后将硝基还原为氨基而引入肽链中。然而，该方法不适用于蛋白质。
