Raghupathi R N, Diwan A M
Department of Zoology, University of Poona, India.
Anal Biochem. 1994 Jun;219(2):356-9. doi: 10.1006/abio.1994.1276.
A modified version of the Lowry protocol for protein measurement is being introduced in which the increment in color due to copper-protein interactions can be directly equated to the concentration of peptide groups (the CO-NH moieties) present in the sample. The method provides an accurate estimation of protein concentrations in the range of 5-40 micrograms, with a sensitivity similar to that of the original assay procedure. It yields hardly any protein-to-protein variation with simple proteins and eliminates the interference by four compounds that commonly affect the Lowry assay. A numerical factor has been calculated that allows conversion of micromoles of peptide groups into actual micrograms of protein present in the sample.
一种用于蛋白质测量的改良版洛瑞法正在被引入，其中由于铜 - 蛋白质相互作用导致的颜色增量可直接等同于样品中存在的肽基团（-CO-NH部分）的浓度。该方法能准确估计5 - 40微克范围内的蛋白质浓度，灵敏度与原始测定方法相似。对于简单蛋白质，它几乎不会产生蛋白质间的差异，并且消除了通常影响洛瑞法的四种化合物的干扰。已经计算出一个数值因子，可将肽基团的微摩尔数转换为样品中实际存在的蛋白质微克数。
