Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, AB, Canada.
Methods Mol Biol. 2022;2579:47-57. doi: 10.1007/978-1-0716-2736-5_4.
Cell cycle analysis is one of the earliest applications in flow cytometry and continues to be highly used to this day. Since the first reported method of Feulgen-DNA staining, cell cycle analysis has continued to grow and mature. With the recent advances in DNA dyes, understanding of additional cell cycle phase markers, and new technologies, cell cycle analysis continues to be a dynamic field within the flow cytometry community. This chapter will give an overview of the current state of cell cycle analysis by flow cytometry.
细胞周期分析是流式细胞术最早的应用之一，至今仍被广泛应用。自首次报道的 Feulgen-DNA 染色方法以来，细胞周期分析不断发展和成熟。随着 DNA 染料的最新进展、对其他细胞周期阶段标志物的深入了解以及新技术的出现，细胞周期分析在流式细胞术领域继续保持着活力。本章将概述当前流式细胞术的细胞周期分析现状。
