通过流式细胞术分析细胞周期
Analysis of Cell Cycle by Flow Cytometry.
作者信息
Maadi Hamid, Soheilifar Mohammad Hasan, Wang Zhixiang
机构信息
Department of Oncology, Cross Cancer Institute, University of Alberta, Edmonton, AB, Canada.
Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran.
出版信息
Methods Mol Biol. 2022;2579:183-195. doi: 10.1007/978-1-0716-2736-5_14.
The cell cycle of a cell is tightly controlled by several regulators. Dysregulation of cell cycle can lead to uncontrolled cell division which is one of the main characteristics of cancer cells. DNA content of a cell is changed during the cell cycle progression and can be measured by flow cytometry. In this chapter, we aim to provide a detailed protocol on how to analyze the cell cycle using flow cytometry.
细胞的细胞周期受到多种调节因子的严格控制。细胞周期失调会导致细胞不受控制地分裂，这是癌细胞的主要特征之一。在细胞周期进程中，细胞的DNA含量会发生变化，可通过流式细胞术进行测量。在本章中，我们旨在提供一份关于如何使用流式细胞术分析细胞周期的详细方案。
Zotero 插件