鉴定 RFX5 作为胃腺癌的预后生物标志物，并与免疫浸润相关。
Identification of RFX5 as prognostic biomarker and associated with immune infiltration in stomach adenocarcinoma.
机构信息
Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, China.
Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, China.
出版信息
Eur J Med Res. 2022 Aug 31;27(1):164. doi: 10.1186/s40001-022-00794-w.
BACKGROUND
Regulatory factor X (RFX) gene family is a series of encodes transcription factors with a highly conserved DNA binding domain. RFXs played a vital role in the development and progression of cancer. However, the significance of RFXs in stomach adenocarcinoma (STAD) has not been fully clarified.
METHODS
Online bioinformatics tools such as GSCALite, Kaplan-Meier Plotter, TIMER, LinkedOmics were used to explore the immunomodulatory function and clinical value of RFXs in STAD.
RESULTS
The mRNA level of RFX1, RFX3, RFX4, RFX5, RFX7 and RFX8 was significantly elevated in STAD tissue versus adjacent normal tissue. We also summarize the copy number variation, single nucleotide variants and drug sensitivity of RFXs in STAD. Prognostic analysis indicated that STAD patients with high RFX5 and RFX7 expression had a better overall survival, first progression, and post-progression survival. Moreover, RFX5 expression was significantly associated with the abundance of immune cells, the expression of immune biomarkers and tumor mutational burden score in STAD. Functional enrichment analysis revealed that RFX5 and its related genes were mainly involved in T cell activation, antigen receptor-mediated signaling pathway, cell adhesion molecules, and Th17 cell differentiation. Validation study further verified the expression and prognosis of RFX5 in STAD. Further univariate and multivariate analyses suggested that pathological stage and RFX5 could be a potential independent prognostic factor for STAD.
CONCLUSIONS
RFX5 was a candidate prognostic biomarker and associated with immune infiltration in STAD.
背景
调节因子 X（RFX）基因家族是一系列编码转录因子的基因，具有高度保守的 DNA 结合结构域。RFXs 在癌症的发生和发展中起着至关重要的作用。然而，RFXs 在胃腺癌（STAD）中的意义尚未完全阐明。
方法
使用 GSCALite、Kaplan-Meier Plotter、TIMER 和 LinkedOmics 等在线生物信息学工具，探讨 RFXs 在 STAD 中的免疫调节功能和临床价值。
结果
与相邻正常组织相比，STAD 组织中 RFX1、RFX3、RFX4、RFX5、RFX7 和 RFX8 的 mRNA 水平显著升高。我们还总结了 STAD 中 RFXs 的拷贝数变异、单核苷酸变异和药物敏感性。预后分析表明，RFX5 和 RFX7 高表达的 STAD 患者总生存率、无进展生存率和进展后生存率更好。此外，RFX5 表达与 STAD 中免疫细胞的丰度、免疫生物标志物的表达和肿瘤突变负荷评分显著相关。功能富集分析表明，RFX5 及其相关基因主要参与 T 细胞激活、抗原受体介导的信号通路、细胞黏附分子和 Th17 细胞分化。验证研究进一步验证了 RFX5 在 STAD 中的表达和预后。进一步的单变量和多变量分析表明，病理分期和 RFX5 可能是 STAD 的一个潜在独立预后因素。
结论
RFX5 是 STAD 的候选预后生物标志物，与免疫浸润有关。
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