School of Pharmacy, China Medical University, Shenyang, China.
Department of Pharmacy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Crit Rev Anal Chem. 2024;54(6):1430-1461. doi: 10.1080/10408347.2022.2116556. Epub 2022 Aug 31.
Anthocyanins (ANCs), a kind of natural pigments, are widely present in food substrates. Evidence has shown that ANCs can promote health in terms of anti-oxidation, anti-tumor, and anti-inflammation. However, the oxidative stability of ANCs limits accurate quantitation and analysis. Therefore, faster, more accurate, and highly sensitive extraction and determination methods are necessary for understanding the role of ANCs in medicine and food. This review presents an updated overview of pretreatment and detection techniques for ANCs in various food substrates since 2015. Liquid-liquid extraction and various green solvent extraction methods, such as accelerated solvents extraction, deep eutectic solvents extraction, ionic liquids extraction, and supercritical fluid extraction, are commonly used pretreatment methods for extraction and purification of ANCs. Liquid chromatography coupled with different detectors (tandem mass spectrometry and UV detectors) and spectrophotometry methods are some of the determination methods for ANC. This study has updated, compared, and discussed different pretreatment and analysis methods. Moreover, the advanced methods and development prospects in this field are comprehensively summarized, which can provide references for further utilization of ANCs.
花色苷（ANCs）是一种天然色素，广泛存在于食品基质中。有证据表明，花色苷具有抗氧化、抗肿瘤和抗炎等促进健康的作用。然而，花色苷的氧化稳定性限制了其准确的定量和分析。因此，为了更好地了解花色苷在医学和食品中的作用，需要更快、更准确、更灵敏的提取和测定方法。本综述介绍了 2015 年以来各种食品基质中花色苷预处理和检测技术的最新进展。液-液萃取和各种绿色溶剂萃取方法，如加速溶剂萃取、深共晶溶剂萃取、离子液体萃取和超临界流体萃取，是花色苷提取和纯化的常用预处理方法。液相色谱与不同检测器（串联质谱和紫外检测器）和分光光度法是花色苷测定的一些方法。本研究对不同的预处理和分析方法进行了更新、比较和讨论。此外，还综合总结了该领域的先进方法和发展前景，为进一步利用花色苷提供了参考。
