College of Materials Science and Engineering, Northeast Forestry University, Hexing Road 26, Harbin 150040, PR China; Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, PR China.
Food Chem. 2019 Oct 30;296:78-85. doi: 10.1016/j.foodchem.2019.05.196. Epub 2019 May 30.
Natural deep eutectic solvents (NADES) have been growing interest as an alternative to the traditional organic solvents. They not only have the merit of high efficiency but also have the possibility to readily applicable to pharmaceutical and food applications. In the present study, NADES with high-speed homogenization and cavitation-burst extraction (HSH-CBE) was performed on fresh mulberry for anthocyanins extraction. The extraction conditions were statistically investigated by Plackett-Burman design (PBD) and Box-Behnken design (BBD). The optimal conditions were obtained as follows: chloride-citric acid-glucose formed a NADES with the mole ratio of 1:1:1, 30% water content, liquid-solid ratio 22 mL/g, homogenization time 60 s, homogenization speed 12,000 rpm, extraction time 30 min, negative pressure -0.08 MPa and extraction two times. The total maximum extraction of anthocyanins reached 6.05 mg/g fresh weight, which was 1.24 folds to those by the traditional organic solvents extraction. Moreover, NADES exhibited higher stability of anthocyanins extraction than traditional organic solvents, which was benefit for the analysis and preservation of anthocyanins. Consequently, this result revealed that the developed method could be taken as a sustainable, green and effective approach for anthocyanins extraction.
天然深共晶溶剂 (NADES) 作为传统有机溶剂的替代品越来越受到关注。它们不仅具有高效的优点，而且有可能很容易应用于制药和食品应用。在本研究中，通过高速均化和空化爆裂提取 (HSH-CBE) 对新鲜桑椹中的花色苷进行 NADES 提取。通过 Plackett-Burman 设计 (PBD) 和 Box-Behnken 设计 (BBD) 对提取条件进行了统计研究。优化条件如下：氯化物-柠檬酸-葡萄糖形成摩尔比为 1:1:1 的 NADES，水含量 30%，液固比 22 mL/g，均质时间 60 s，均质速度 12,000 rpm，提取时间 30 min，负压-0.08 MPa，提取两次。花色苷的总最大提取量达到 6.05 mg/g 鲜重，是传统有机溶剂提取法的 1.24 倍。此外，NADES 显示出比传统有机溶剂更高的花色苷提取稳定性，这有利于花色苷的分析和保存。因此，该结果表明，所开发的方法可以作为花色苷提取的一种可持续、绿色和有效的方法。
