研究藏红花花瓣水醇提取物对多囊卵巢综合征小鼠模型卵巢卵泡、炎症标志物及抗氧化酶的影响。

Investigating the Effect of Crocus sativus (Saffron) Petal Hydro-alcoholic Extract on Ovarian Follicle, Inflammatory Markers, and Antioxidant Enzymes in Mice Model of Polycystic Ovary Syndrome.

作者信息

Moshfegh Fazeleh, Zafar Balanejad Saeedeh, Shahrokhabady Khadige, Attaranzadeh Armin

机构信息

Department of Biology, Faculty of Basic Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

Department of Milad IVF, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

出版信息

J Reprod Infertil. 2022 Jan-Mar;23(1):18-25. doi: 10.18502/jri.v23i1.8447.

DOI:10.18502/jri.v23i1.8447

PMID:36045883

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9361724/

Abstract

BACKGROUND

Saffron petals have traditionally been used to treat a variety of diseases, such as gynecological diseases, primary dysmenorrhea, and premenstrual syndrome. Polycystic ovary syndrome (PCOS) is a kind of gynecological disease that causes infertility, menopausal and urogenital disorders and saffron petals seem to be an efficient treatment for such disorders.

METHODS

NMRI mice (total=60, each group n=12) were divided into control, PCOS, and the treatment groups. PCOS and treatment groups were injected with testosterone enanthate (TE=1 ). After 4 weeks, the treatment group was treated with Saffron Petal Extract (SPE) for 14 days. Ovary and blood samples were collected for histological and serological analyses, and expression of NF-κB, NF-κB p65, and IκB genes was analyzed. Finally, data were analyzed using one-way ANOVA (p<0.05).

RESULTS

In this study, the number of corpus luteum decreased in PCOS mice (p<0.001) but increased with SPE treatment (p<0.05, p<0.01, p<0.001). The number of cystic follicles increased in PCOS mice (p<0.001), but decreased with SPE treatment (p<0.05, p<0.001). TNFα, IL1ß, IL6, IL18, and CRP levels increased in PCOS mice (p<0.001), but decreased following SPE treatment (p<0.05, p<0.001). Glutathione (GSH) and glutathione S-transferase (GST) serum levels decreased in PCOS mice (p<0.001), but increased with SPE treatment (p<0.05, p<0.001). The transcriptional level (s) of NF-κB, NF-κB p65, IκB genes changed in PCOS condition (p<0.001), and were regulated by SPE treatment (p<0.05, p<0.01 and p<0.001).

CONCLUSION

The present study shows that SPE improved the PCOS symptoms in mice via increasing antioxidant factors and reducing inflammatory markers in serum.

摘要

背景

传统上藏红花花瓣被用于治疗多种疾病，如妇科疾病、原发性痛经和经前综合征。多囊卵巢综合征（PCOS）是一种导致不孕、更年期和泌尿生殖系统紊乱的妇科疾病，而藏红花花瓣似乎对这类疾病有有效的治疗作用。

方法

将NMRI小鼠（共60只，每组n = 12）分为对照组、PCOS组和治疗组。PCOS组和治疗组注射庚酸睾酮（TE = 1）。4周后，治疗组用藏红花花瓣提取物（SPE）治疗14天。采集卵巢和血液样本进行组织学和血清学分析，并分析NF-κB、NF-κB p65和IκB基因的表达。最后，使用单因素方差分析（p < 0.05）对数据进行分析。

结果

在本研究中，PCOS小鼠的黄体数量减少（p < 0.001），但经SPE治疗后增加（p < 0.05、p < 0.01、p < 0.001）。PCOS小鼠的囊性卵泡数量增加（p < 0.001），但经SPE治疗后减少（p < 0.05、p < 0.001）。PCOS小鼠的TNFα、IL1ß、IL6、IL18和CRP水平升高（p < 0.001），但经SPE治疗后降低（p < 0.05、p < 0.001）。PCOS小鼠的谷胱甘肽（GSH）和谷胱甘肽S-转移酶（GST）血清水平降低（p < 0.001），但经SPE治疗后升高（p < 0.05、p < 0.001）。PCOS状态下NF-κB、NF-κB p65、IκB基因的转录水平发生变化（p < 0.001），并受SPE治疗调控（p < 0.05、p < 0.01和p < 0.001）。