Yang Peng-Hui, Jin Li-Jun, Liao Jie, Shao Xin, Cheng Jun-Yun, Li Lu, Lu Xiao-Yan, Fan Xiao-Hui
Department of Chinese Medicine Science & Engineering, College of Pharmaceutical Sciences, Zhejiang University Hangzhou 310058, China.
Department of Chinese Medicine Science & Engineering, College of Pharmaceutical Sciences, Zhejiang University Hangzhou 310058, China Innovation Center in Zhejiang University, State Key Laboratory of Component-based Chinese Medicine Hangzhou 310058, China.
Zhongguo Zhong Yao Za Zhi. 2022 Aug;47(15):3977-3985. doi: 10.19540/j.cnki.cjcmm.20220601.702.
As one of the most advanced technologies, single-cell omics technology develops rapidly in recent years. Based on different technical strategies, it enables unbiased and high-throughput access to multiple omics information at single-cell resolution. So far, single-cell omics technology, by virtue of its great powder in resolving tissue heterogeneity, has become a revolutionary tool to deeply understand the functional structure of tissues, reveal complex disease processes, and elucidate drug mechanisms of action. In view of the technical challenges in deconstructing the complexity of Chinese medicine and clarifying the modern scientific connotation of traditional Chinese medicine(TCM) theory, single-cell omics technology has huge application potential in the discovery of pharmacodynamic substances, construction of action networks, and elucidation of integrated regulatory mechanisms, which brings new opportunities for modern research in TCM. The present study briefly introduced three representative single-cell omics technologies, i.e., single-cell transcriptome sequencing, spatial transcriptomics, and single-cell multimodal omics, and their main application patterns. On this basis, an outlook was proposed on the strategies and applications for modern research in TCM using single-cell omics technology.
作为最先进的技术之一，单细胞组学技术近年来发展迅速。基于不同的技术策略，它能够以单细胞分辨率无偏倚且高通量地获取多种组学信息。到目前为止，单细胞组学技术凭借其在解析组织异质性方面的强大能力，已成为深入了解组织功能结构、揭示复杂疾病过程以及阐明药物作用机制的革命性工具。鉴于解构中药复杂性和阐明中医理论现代科学内涵方面存在技术挑战，单细胞组学技术在药效物质发现、作用网络构建以及综合调控机制阐释等方面具有巨大的应用潜力，为中医现代研究带来了新机遇。本研究简要介绍了三种具有代表性的单细胞组学技术，即单细胞转录组测序、空间转录组学和单细胞多组学，以及它们的主要应用模式。在此基础上，对利用单细胞组学技术进行中医现代研究的策略和应用提出了展望。
