Xin Ping, Kuang Hai-Xue, Li Xiao-Liang, Wang Yu, Zhang Ben-Mei, Bu He, Wang Zhi-Bin, Meng Yong-Hai, Wang Yan-Hong, Wang Qiu-Hong
Key Laboratory of Chinese Materia Medica, Ministry of Education, Heilongjiang Key Laboratory of Traditional Chinese Medicine Pharmacodynamic Material, Heilongjiang University of Chinese Medicine, Harbin 150040, China.
Guangdong Pharmaceutical University, Guangzhou 510006, China.
Zhongguo Zhong Yao Za Zhi. 2018 Mar;43(5):904-912. doi: 10.19540/j.cnki.cjcmm.20180118.002.
There is no doubt that the traditional Chinese medicine(TCM) is effective, practical and scientific after it was used for thousands of years. However, the mechanisms of action of many TCM are still unclear because of their multi-component, multi-target and multi-level features, which hinder the modernization and internationalization of the TCM. Proteomics is to analyze the composition and activity of intracellular proteins which are changing dynamically from a holistic perspective. It is consistent with the holistic and dynamic views of the TCM and brings about the hope of clarifying the mechanism of action of the TCM. In recent years, great progress has been made in the application of proteomics to determine the mechanism of the TCM. This article introduced the core technologies of proteomics and systematically summarized the applications of proteomics in the study of the mechanism of the Chinese medicinal formulae, single Chinese medicine and monomeric compounds from the TCM to provide innovative ideas and methods for reference.
毫无疑问，中药在经过数千年的应用后是有效、实用且科学的。然而，由于许多中药具有多成分、多靶点和多层次的特点，其作用机制仍不明确，这阻碍了中药的现代化和国际化。蛋白质组学是从整体角度分析细胞内动态变化的蛋白质组成和活性。它与中医的整体观和动态观相一致，为阐明中药作用机制带来了希望。近年来，蛋白质组学在确定中药作用机制方面取得了很大进展。本文介绍了蛋白质组学的核心技术，并系统总结了蛋白质组学在中药复方、单味中药及中药单体化合物作用机制研究中的应用，以供参考，提供创新思路和方法。
