Yun Junseob, Lee Jungwon, Kim Sungtae, Koo Ki-Tae, Seol Yang-Jo, Lee Yong-Moo
Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea.
One-Stop Specialty Center, Seoul National University Dental Hospital, Seoul, Korea.
J Periodontal Implant Sci. 2022 Aug;52(4):312-324. doi: 10.5051/jpis.2104700235.
The role of hard-type crosslinked hyaluronic acid (HA) with particulate bone substitutes in bone regeneration for combined inlay-onlay grafts has not been fully investigated. We aimed to evaluate the effect of hard-type crosslinked HA used with bone substitute in terms of new bone formation and space maintenance.
A 15-mm-diameter round defect was formed in the calvaria of 30 New Zealand White rabbits. All animals were randomly assigned to 1 of 3 groups: the control group (spontaneous healing without material, n=10), the biphasic calcium phosphate (BCP) graft group (BCP, n=10), and the BCP graft with HA group (BCP/HA, n=10). The animals were evaluated 4 and 12 weeks after surgery. Half of the animals from each group were sacrificed at 4 and 12 weeks after surgery. Samples were evaluated using micro-computed tomography, histology, and histomorphometry.
The BCP group showed higher bone volume/tissue volume (BV/TV) values than the control and BCP/HA groups at both 4 and 12 weeks. The BCP and BCP/HA groups showed higher bone surface/tissue volume (BS/TV) values than the control group at both 4 and 12 weeks. The BCP group showed higher BS/TV values than the control and BCP/HA groups at both 4 and 12 weeks. No statistically significant difference in newly formed bone was found among the 3 groups at 4 weeks. The BCP group showed significantly higher new bone formation than the BCP/HA group at 12 weeks.
Hard-type crosslinked HA did not show a positive effect on new bone formation and space maintenance. The negative effect of hard-type crosslinked HA may be due to the physical properties of HA that impede osteogenic potential.
硬质交联透明质酸（HA）与颗粒状骨替代物在联合嵌体-覆盖移植骨再生中的作用尚未得到充分研究。我们旨在评估硬质交联HA与骨替代物联合使用在新骨形成和空间维持方面的效果。
在30只新西兰白兔的颅骨上形成一个直径15毫米的圆形缺损。所有动物随机分为3组中的1组：对照组（不使用材料自然愈合，n = 10）、双相磷酸钙（BCP）移植组（BCP，n = 10）和BCP与HA联合移植组（BCP/HA，n = 10）。术后4周和12周对动物进行评估。每组一半的动物在术后4周和12周处死。使用微型计算机断层扫描、组织学和组织形态计量学对样本进行评估。
在4周和12周时，BCP组的骨体积/组织体积（BV/TV）值均高于对照组和BCP/HA组。在4周和12周时，BCP组和BCP/HA组的骨表面积/组织体积（BS/TV）值均高于对照组。在4周和12周时，BCP组的BS/TV值均高于对照组和BCP/HA组。4周时，3组之间新形成的骨没有统计学上的显著差异。12周时，BCP组的新骨形成明显高于BCP/HA组。
硬质交联HA对新骨形成和空间维持没有显示出积极作用。硬质交联HA的负面影响可能是由于HA的物理性质阻碍了成骨潜力。
