College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, China.
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
J Agric Food Chem. 2022 Sep 14;70(36):11429-11440. doi: 10.1021/acs.jafc.2c04529. Epub 2022 Sep 1.
Coss. is a malignant weed in wheat fields in China, its herbicide resistance has been threatening crop production. This study identified one mesosulfuron-methyl-resistant(R) population, JJMHN2018-05 (R), without target resistance mutations. To fully understand the resistance mechanism, non-target site resistance was investigated by using transcriptome sequencing combined with a reference genome. Results showed that the cytochrome P450 monooxygenase (P450) inhibitor malathion significantly increased the mesosulfuron-methyl sensitivity in R plants, and greater herbicide-induced glutathione S-transferase (GST) activity was also confirmed. Liquid chromatography with tandem mass spectrometry analysis further supported the enhanced mesosulfuron-methyl metabolism in R plants. Gene expression data analysis and qRT-PCR validation indicated that eight P450s, six GSTs, two glycosyltransferases (GTs), four peroxidases, and one aldo-keto reductase (AKRs) stably upregulated in R plants. This research demonstrates that the P450s and GSTs involved in enhanced mesosulfuron-methyl metabolism contribute to mesosulfuron-methyl resistance in and identifies potential contributors from metabolic enzyme families.
节节麦是中国麦田中的恶性杂草，其抗药性一直威胁着作物的生产。本研究鉴定了一个无靶标抗性突变的啶磺草胺抗（R）种群，JJMHN2018-05（R）。为了充分了解其抗性机制，我们使用转录组测序结合参考基因组研究了非靶标抗性。结果表明，拟除虫菊酯抑制剂马拉硫磷显著提高了 R 植株对啶磺草胺的敏感性，并且证实了更强的除草剂诱导谷胱甘肽 S-转移酶（GST）活性。液质联用分析进一步支持了 R 植株中啶磺草胺的增强代谢。基因表达数据分析和 qRT-PCR 验证表明，R 植株中 8 个细胞色素 P450 单加氧酶（P450）、6 个谷胱甘肽 S-转移酶（GST）、2 个糖基转移酶（GT）、4 个过氧化物酶和 1 个醛酮还原酶（AKRs）稳定地上调。本研究表明，参与增强啶磺草胺代谢的 P450s 和 GSTs 导致了对啶磺草胺的抗性，并鉴定出了代谢酶家族中潜在的贡献者。
