State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, Jiangxi 330047, China.
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611134, China.
J Agric Food Chem. 2022 Sep 14;70(36):11224-11235. doi: 10.1021/acs.jafc.2c02334. Epub 2022 Sep 1.
The present study investigated the mechanism underlying the impact of hesperidin (HES) on nonalcoholic fatty liver (NAFLD). C57BL/6J male mice were administered a low-fat diet, high-fat diet (HFD), or HFD plus 0.2% (wt/wt) HES (HFD + HES) diet. After 16 weeks of intervention, the mice in the HFD+HES group showed a lower final body weight and liver weight and improved serum lipid profiles when compared with the HFD group. Alleviation of liver dysfunction induced by HFD was observed in HES-fed mice, and the expression of genes involved in lipid metabolism was also altered. Moreover, HES changed the composition of the intestinal microbiota and enriched specific genera such as . Liver metabolomics analysis indicated that HES enhanced the abundance of metabolites in arginine-related as well as mitochondrial oxidation-related pathways, and these metabolites were predicted to be positively correlated with the gut genera enriched by HES. Together, these results indicate that HFD-fed mice supplemented with HES showed a markedly regulated hepatic metabolism concurrent with shifts in specific gut bacteria.
本研究旨在探讨橙皮苷（HES）对非酒精性脂肪肝（NAFLD）影响的作用机制。将 C57BL/6J 雄性小鼠给予低脂饮食、高脂饮食（HFD）或 HFD 加 0.2%（wt/wt）HES（HFD+HES）饮食。干预 16 周后，与 HFD 组相比，HFD+HES 组小鼠的最终体重和肝重较低，血清脂质谱得到改善。在 HES 喂养的小鼠中观察到 HFD 诱导的肝功能障碍得到缓解，并且参与脂质代谢的基因的表达也发生了改变。此外，HES 改变了肠道微生物群的组成，并富集了特定的属，如. 肝脏代谢组学分析表明，HES 增强了精氨酸相关和线粒体氧化相关途径中代谢物的丰度，这些代谢物被预测与 HES 富集的肠道属呈正相关。综上所述，这些结果表明，补充 HES 的 HFD 喂养小鼠表现出明显的肝脏代谢调节，同时特定肠道细菌发生变化。
