Chaudhury Debajit, Sen Utsav, Biswas Siddhartha, Shenoy P Sudheer, Bose Bipasha
Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya (Deemed to be University), University Road, Derlakatte, Mangalore, Karnataka, 575018, India.
Department of Onco-Pathology, Yenepoya Medical College, Yenepoya (Deemed to be University), University Road, Derlakatte, Mangalore, Karnataka, 575018, India.
Biol Trace Elem Res. 2023 Jun;201(6):2927-2941. doi: 10.1007/s12011-022-03398-x. Epub 2022 Sep 1.
The dose contributed from thoron (Rn) and its progeny has been neglected in the dose assessment because of its short half-life (t = 55.6 s) and generally low concentrations. Recently, concentrations of Rn gas and its progeny were found to be pronounced in the traditional residential dwellings in China, on beaches of India and in other countries. Accordingly, we investigated the biological effects of thoron (Rn) decay products in various mouse organs, succeeding inhalation of thoron gas in BALB/c mouse. We investigated the biological effects upon thoron inhalation on mouse organs with a focus on oxidative stress. These mice were divided into (4 random groups): sham inhalation, thoron inhalation for 1, 4 and 10 days. Various tissues (lung, liver and kidney) were then collected after the time points and subjected to various biochemical analyses. Immediately after inhalation, mouse tissues were excised for gamma spectrometry and 72 h post inhalation for biochemical assays. The gamma spectrometry counts and its subsequent calculation of the equivalent dose showed varied distribution in the lung, liver and kidney. Our results suggest that acute thoron inhalation showed a differential effect on the antioxidant function and exerted pathophysiological alterations via oxidative stress in organs at a higher dose. These findings suggested that thoron inhalation could alter the redox state in organs; however, its characteristics were dependent on the total redox system of the organs as well as the thoron concentration and inhalation time.
由于钍射气（Rn）及其子体的半衰期较短（t = 55.6秒）且浓度通常较低，因此在剂量评估中其贡献的剂量被忽略了。最近，在中国的传统民居、印度的海滩及其他国家，发现Rn气体及其子体的浓度显著。因此，在BALB/c小鼠成功吸入钍射气后，我们研究了钍射气（Rn）衰变产物在小鼠各个器官中的生物学效应。我们重点研究了吸入钍射气对小鼠器官的生物学效应，特别是氧化应激方面。这些小鼠被分为4个随机组：假吸入组、吸入钍射气1天组、吸入钍射气4天组和吸入钍射气10天组。在不同时间点后收集各种组织（肺、肝和肾），并进行各种生化分析。吸入后立即切除小鼠组织进行伽马能谱分析，吸入后72小时进行生化检测。伽马能谱计数及其等效剂量的后续计算显示，肺、肝和肾中的分布各不相同。我们的结果表明，急性吸入钍射气对抗氧化功能有不同影响，并在较高剂量下通过氧化应激在器官中引起病理生理改变。这些发现表明，吸入钍射气会改变器官中的氧化还原状态；然而，其特征取决于器官的总氧化还原系统以及钍射气浓度和吸入时间。
