Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University, Guiyang 550005, China.
Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region, College of Biology and Environmental Engineering, Guiyang University, Guiyang 550005, China.
Int J Biol Macromol. 2022 Nov 1;220:1212-1220. doi: 10.1016/j.ijbiomac.2022.08.162. Epub 2022 Aug 29.
The peptidoglycan recognition protein (PGRP), an important pattern recognition receptor of insects, is significant for reducing innate immunity and effective pest control. We cloned four PGRP genes (LsPGRP-LB, LsPGRP-LB1, LsPGRP-LE, and LsPGRP-SC2) from the cigarette beetle, Lasioderma serricorne (Fabricius), which encoded proteins of 216, 197, 317, and 190 amino acids, respectively. Three LsPGRPs were predominantly expressed in the larval and pupal stages, whereas LsPGRP-LE displayed high expression in adults. All the four LsPGRPs genes were highly expressed in the midgut and integument. Pathogen inoculation revealed that the four LsPGRPs actively responded to Escherichia coli and its peptidoglycan. The transcription levels of LsPGRP-LE and LsPGRP-SC2 increased significantly after Staphylococcus aureus stimulation. RNA interference-mediated knockdown of the four LsPGRPs led to increased larval mortality when challenged by E. coli, and the expression of four antimicrobial peptide genes (LsCole, LsAtt2, LsDef1 and LsDef2) had a significant decrease. Higher mortality and lower AMP expression were also observed in L. serricorne under S. aureus infection after silencing LsPGRP-LE and LsPGRP-SC2. Our results suggest that the four LsPGRP genes play important and distinct regulatory roles in the antibacterial defense response of L. serricorne.
肽聚糖识别蛋白（PGRP）是昆虫重要的模式识别受体，对于降低先天免疫和有效控制害虫至关重要。我们从烟草甲（Lasioderma serricorne）克隆了四个 PGRP 基因（LsPGRP-LB、LsPGRP-LB1、LsPGRP-LE 和 LsPGRP-SC2），它们分别编码 216、197、317 和 190 个氨基酸的蛋白质。三个 LsPGRPs 在幼虫和蛹期主要表达，而 LsPGRP-LE 在成虫期表达量较高。四个 LsPGRPs 基因在中肠和表皮中均高度表达。病原体接种表明，四个 LsPGRPs 对大肠杆菌及其肽聚糖均有积极的响应。金黄色葡萄球菌刺激后，LsPGRP-LE 和 LsPGRP-SC2 的转录水平显著增加。四种抗菌肽基因（LsCole、LsAtt2、LsDef1 和 LsDef2）的表达在大肠杆菌攻击后，四个 LsPGRPs 的 RNAi 介导的敲低导致幼虫死亡率显著增加。在沉默 LsPGRP-LE 和 LsPGRP-SC2 后，金黄色葡萄球菌感染的烟草甲死亡率更高，抗菌肽表达量更低。我们的研究结果表明，四个 LsPGRP 基因在烟草甲的抗菌防御反应中发挥重要且不同的调节作用。
