He Li, Zhang Chao, Yang Hong, Ding Bo, Yang Han-Zhi, Zhang Sen-Wen
Guizhou Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region, Institute of Entomology, Guizhou University, Guiyang 550025, China.
Insects. 2023 Mar 9;14(3):275. doi: 10.3390/insects14030275.
The insect Toll receptor is one of the key members of the Toll signaling pathway, which plays an indispensable role in insect resistance to pathogen infection. Herein, we cloned and characterized five Toll receptor genes from (Sulzer), which were found to be highly expressed in the first-instar nymphs and adults (both wingless and winged) at different developmental stages. Expressions of MpToll genes were highest in the head, followed by the epidermis. High transcription levels were also found in embryos. Expressions of these genes showed different degrees of positive responses to infection by and . The expression of - and significantly increased after infection with , whereas the expression of , , -, and continuously increased after infection with . RNA interference-mediated suppressed expression of these genes resulted in a significant increase in the mortality of after infection with the two bacterial species compared with that in the control group. These results suggest that MpToll genes play vital roles in the defense response of against bacteria.
昆虫Toll受体是Toll信号通路的关键成员之一，在昆虫抵抗病原体感染中发挥着不可或缺的作用。在此，我们从（苏尔泽）中克隆并鉴定了五个Toll受体基因，发现它们在不同发育阶段的一龄若虫和成虫（无翅和有翅）中高表达。MpToll基因在头部表达最高，其次是表皮。在胚胎中也发现了高转录水平。这些基因的表达对 和 的感染表现出不同程度的阳性反应。感染 后， - 和 的表达显著增加，而感染 后， 、 、 - 和 的表达持续增加。与对照组相比，RNA干扰介导的这些基因表达抑制导致感染两种细菌后 的死亡率显著增加。这些结果表明，MpToll基因在 对细菌的防御反应中起着至关重要的作用。
