Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China.
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources (Shanghai Ocean University), Ministry of Education, 201306, China; National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, 201306, China.
Dev Comp Immunol. 2022 Dec;137:104522. doi: 10.1016/j.dci.2022.104522. Epub 2022 Aug 30.
As a non-coding RNA with regulatory functions, microRNAs(miRNAs) can regulate gene expression and participate in a variety of physiological and pathological processes. In recent years, although there have been many studies on miRNA, the regulation mechanisms of miRNA in teleost fish have not been fully elucidated. In this study, it was first predicted that MITA is the target of miR-27c through bioinformatics, and it was confirmed by dual fluorescence experiments. Then we found that miR-27c can inhibit the expression of MITA at the mRNA and protein levels, thereby promoting the NF-κB or IRF3 pathway. It is speculated that miR-27c plays an important role in the innate immunity of teleost fish. This study will help to further understand miRNAs regulatory mechanism in teleost fish.
作为一种具有调节功能的非编码 RNA，microRNAs（miRNAs）可以调节基因表达并参与多种生理和病理过程。近年来，尽管已经有许多关于 miRNA 的研究，但 miRNA 在硬骨鱼类中的调控机制尚未完全阐明。在这项研究中，首先通过生物信息学预测 MITA 是 miR-27c 的靶标，并通过双荧光实验得到了验证。然后我们发现 miR-27c 可以在 mRNA 和蛋白质水平上抑制 MITA 的表达，从而促进 NF-κB 或 IRF3 通路。推测 miR-27c 在硬骨鱼类的先天免疫中发挥重要作用。本研究将有助于进一步了解 miRNAs 在硬骨鱼类中的调控机制。
